目的:本研究旨在确定133例严重急性呼吸综合征冠状病毒2(SARS-CoV-2)RT-PCR阳性患者中弓形虫感染与COVID-19结局之间的可能关联。在伊玛目霍梅尼医院住院,Sari,Mazandaran省,伊朗北部,2020年8月至11月。
方法:使用问卷收集在伊朗国家弓形虫病登记中心(INRCT)登记的患者的基线数据。此外,从每位患者身上采集血样以检测抗T。使用酶联免疫吸附测定(ELISA)和常规PCR方法,分别。根据多重多项逻辑回归模型,指出了与COVID-19严重程度和结局相关的变量。
结果:在通过RT-PCR纳入COVID-19INRCT的133例患者中,50(37.59%),52(39.1%),31人(23%)患有轻度疾病,中度,和严重的COVID-19。57.1%的死亡患者患有严重的COVID-19,而在其他结局患者中,重症COVID-19患者仅占18.60%(P<0.05)。反T.109/133例(81.95%)患者检测到gondiiIgG,差异无统计学意义(P>0.05)。在具有负面和正面抗T的人群中弓形虫IgG,重症COVID-19患者分别为2例(8.30%)和29例(26.60%)(P>0.05)。T.弓形虫DNA和抗T.在任何患者中均未发现刚地IgM。此外,所有死亡均发生在中度或重度COVID-19和抗T阳性的患者中。弓形虫IgG。
结论:据我们所知,这是第一项基于注册的关于COVID-19患者弓形虫感染的研究。我们的数据显示,不同严重程度的COVID-19隐匿性弓形虫感染率高。然而,潜伏弓形虫感染与COVID-19严重程度和预后之间没有显著关系。因此,在世界不同地理区域进行多中心研究可以更好地理解这种关系。
OBJECTIVE: This study aimed to determine the possible association between Toxoplasma gondii infection and COVID-19 outcomes among 133 patients with an RT-PCR-positive test for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), hospitalized at Imam Khomeini Hospital, Sari, Mazandaran Province, northern Iran, during August to November 2020.
METHODS: A questionnaire was used to collect baseline data from the patients who were registered to the Iranian National Registry Center for Toxoplasmosis (INRCT). Also, blood samples were taken from each patient for detecting anti-T. gondii antibodies and T. gondii DNA using enzyme-linked immunosorbent assay (ELISA) and conventional-PCR methods, respectively. Variables related to the COVID-19 severity and outcomes were indicated based on multiple multinomial logistic regression models.
RESULTS: Of 133 patients enrolled in the INRCT with COVID-19 through RT-PCR, 50 (37.59%), 52 (39.1%), and 31 (23%) suffered from mild, moderate, and severe COVID-19, respectively. 57.1% of the patients who died had severe COVID-19, while among those with other outcomes, only 18.60% had severe COVID-19 (P < 0.05). Anti-T. gondii IgG was detected in 109/133 (81.95%) patients, which was not statistically significant (P > 0.05). Among those with negative and positive anti-T. gondii IgG, 2 (8.30%) and 29 (26.60%) had severe COVID-19, respectively (P > 0.05). T. gondii DNA and anti-T. gondii IgM were not found in any of the patients. Moreover, all deaths occurred in those with moderate or severe COVID-19 and a positive anti-T. gondii IgG.
CONCLUSIONS: To our knowledge, this is the first registry-based study concerning T. gondii infection among patients with COVID-19. Our data show the high rate of latent T. gondii infection among COVID-19 with different severity. However, there is no significant relationship between latent T. gondii infection and COVID-19 severity and outcomes. Thus, conducting multicenter studies in different geographic regions of the world could offer a better understanding of this relationship.