目的:评估使用FreeStyleLibre(一种连续血糖监测系统[CGMS])瞬时连续监测成年马间质葡萄糖的可行性,并检查该系统在接受葡萄糖-胰岛素联合测试(CGIT)的马匹中的适用性和准确性。
方法:使用2×2阶乘统计模型分析实验室测量和连续葡萄糖监测系统(CGMS)读数,并随时间重复测量。该分析评估了测试的效果(因素1),组(因素2),以及它们的相互作用(测试×组,测试×时间,和组×时间)。对血糖值进行Pearson相关分析。使用t检验进行平均值比较,通过Bland-Altman方法评估技术之间的一致性,95%的置信区间。
方法:与兽医学校合作对私人马场进行实地研究。
方法:根据体况评分(BCS)将10匹健康种马分为两组。第1组(G1,n=5)由BCS为5或6的非肥胖马组成,而第2组(G2,n=5)由BCS为7或更高的肥胖马组成。
方法:将CGMS传感器连接到每匹马颈部近三分之一的背外侧。在传感器固定后长达7天的不同时间点比较实验室血糖测量和CGMS间质葡萄糖读数。肥胖马也在第4天提交给CGIT。
结果:使用CGMS和酶法对G1和G2马的葡萄糖测量结果进行的比较分析显示出明显的群体×时间相互作用(P<0.001)和时间效应(P<0.001)。组间没有检测到相互作用(P=0.45),试验(P=0.62),分组和检验(P=0.28),或时间和检验(P=0.92)。在G1和G2中,所有时间点(T0-T5)的测试均显着相关(r=0.84和P=0.00)。尽管CGMS检测血糖快速变化的时间延迟很小,但使用不同方法获得的葡萄糖值之间的一致性还是很好的。
结论:得出的结论是,CGMS可用于间接评估血糖状态(即,基于间质葡萄糖测量)在提交给CGIT的非肥胖和肥胖成年马中。
OBJECTIVE: To evaluate the feasibility of using the FreeStyle Libre (a continuous glucose monitoring system [CGMS]) for instantaneous continuous monitoring of interstitial glucose in adult horses and examine the applicability and accuracy of this system in horses submitted to combined glucose-insulin test (CGIT).
METHODS: Laboratory measurements and continuous glucose monitoring system (CGMS) readings were analyzed using a 2 × 2 factorial statistical model with repeated measures over time. This analysis assessed the effects of the test (factor 1), group (factor 2), and their interactions (test × group, test × time, and group × time). Pearson\'s correlation analysis was applied to blood glucose values. Mean comparisons were conducted using the t-test, and agreement between techniques was assessed via the Bland-Altman method, with a 95% confidence interval.
METHODS: Field study on private horse farms in association with a veterinary school.
METHODS: Ten healthy stallions were assigned to one of two groups based on their body condition scores (BCS). Group 1 (G1, n = 5) consisted of nonobese horses with a BCS of 5 or 6, while Group 2 (G2, n = 5) consisted of obese horses with a BCS of 7 or higher.
METHODS: A CGMS sensor was attached to the dorsolateral aspect of the proximal one third of each horse\'s neck. Laboratory blood glucose measurements and CGMS interstitial glucose readings were compared at different time points for up to 7 days after sensor fixation. Obese horses were also submitted to CGIT on Day 4.
RESULTS: A comparative analysis of glucose measurements obtained in G1 and G2 horses using the CGMS and enzymatic methods revealed significant group × time interactions (P < 0.001) and time effects (P < 0.001). No interactions were detected between group (P = 0.45), test (P = 0.62), group and test (P = 0.28), or time and test (P = 0.92). In G1 and G2, tests were significantly correlated (r = 0.84 and P = 0.00) at all time points (T0-T5). Agreement between the glucose values obtained using different methods was excellent despite a small time delay in CGMS detection of rapid changes in blood glucose.
CONCLUSIONS: It was concluded that the CGMS can be used for indirect assessment of glycemic status (ie, based on interstitial glucose measurements) in nonobese and obese adult horses submitted to CGIT.