PDF(Pubmed)
Abstract：
Obesity is a complex disease associated with augmented risk of metabolic disorder development and cellular dysfunction in various species. The goal of the present study was to investigate the impacts of obesity on the metabolic health of old mares as well as test the ability of diet supplementation with either a complex blend of nutrients designed to improve equine metabolism and gastrointestinal health or L-carnitine alone to mitigate negative effects of obesity. Mares (n = 19, 17.9 ± 3.7 years) were placed into one of three group: normal-weight (NW, n = 6), obese (OB, n = 7) or obese fed a complex diet supplement for 12 weeks (OBD, n = 6). After 12 weeks and completion of sample collections, OB mares received L-carnitine alone for an additional 6 weeks. Obesity in mares was significantly associated with insulin dysregulation, reduced muscle mitochondrial function, and decreased skeletal muscle oxidative capacity with greater ROS production when compared to NW. Obese mares fed the complex diet supplement had better insulin sensivity, greater cell lipid metabolism, and higher muscle oxidative capacity with reduced ROS production than OB. L-carnitine supplementation alone did not significantly alter insulin signaling, but improved lipid metabolism and muscle oxidative capacity with reduced ROS. In conclusion, obesity is associated with insulin dysregulation and altered skeletal muscle metabolism in older mares. However, dietary interventions are an effective strategy to improve metabolic status and skeletal muscle mitochondrial function in older mares.
摘要：
肥胖是一种复杂的疾病，与各种物种中代谢紊乱发展和细胞功能障碍的风险增加有关。本研究的目的是研究肥胖对老母马代谢健康的影响，以及测试饮食补充与旨在改善马代谢和胃肠道健康的复杂营养素混合物或单独使用L-肉碱的能力减轻肥胖的负面影响。将母马（n=19，17.9±3.7岁）分为三组之一：正常体重（NW，n=6),肥胖(OB，n=7）或肥胖喂养复杂的饮食补充剂12周（OBD，n=6)。在12周完成样本采集后，OB母马单独接受L-肉碱再接受6周。母马的肥胖与胰岛素失调显著相关，肌肉线粒体功能降低，与NW相比，骨骼肌氧化能力降低，ROS产生更大。饲喂复杂饮食补充剂的肥胖母马具有更好的胰岛素敏感性，大细胞脂质代谢，与OB相比，肌肉氧化能力更高，ROS产生减少。单独补充L-肉碱并没有显著改变胰岛素信号，但随着ROS的减少，脂质代谢和肌肉氧化能力得到改善。总之,肥胖与老年母马的胰岛素失调和骨骼肌代谢改变有关。然而,饮食干预是改善老年母马代谢状态和骨骼肌线粒体功能的有效策略。
