■双调蛋白(AR)是一种类似于表皮生长因子(EGF)的生长因子,在不同的细胞中发挥各种功能。然而,目前尚无关于AR在人卵母细胞中的作用的系统研究或报道.本研究旨在探讨人未成熟卵母细胞在体外成熟(IVM)和体外受精(IVF)过程中的AR在实现更好的胚胎发育中的作用,并为开发卵丘卵母细胞复合物(COCs)特异性的授精前培养基提供依据。
■首先,我们检测了接受常规IVF的患者卵泡液中AR的浓度,并探讨了AR水平与卵母细胞成熟和随后的胚胎发育之间的相关性.第二,将AR添加到IVM培养基中培养未成熟卵母细胞,并研究AR是否可以改善IVM的作用。最后,我们率先使用补充AR的受精培养基进行COC的授精前培养,以探索AR的参与是否可以促进IVF卵母细胞的成熟和受精,以及随后的胚胎发育。
■共检查了609个FF样品,AR水平与囊胚形成呈正相关。在我们的IVM研究中,未成熟卵母细胞的发育潜力和IVM率,以及添加AR组的IVM卵母细胞的受精率,与对照组比较,差异均有统计学意义(均P<0.05)。只有IVM-50组的囊胚形成率明显高于对照组(P<0.05)。在最后的试管婴儿研究中,成熟,受精,高质量的胚胎,囊胚形成,添加AR组的囊胚率均显著高于对照组(均P<0.05)。
■FF中的AR水平与胚泡形成呈正相关,和AR参与COCs的授精前培养可以有效改善IVF的实验室结局。此外,最佳浓度为50ng/ml时,AR可直接促进人未成熟卵母细胞的体外成熟和发育潜能。
UNASSIGNED: Amphiregulin (AR) is a growth factor that resembles the epidermal growth factor (EGF) and serves various functions in different cells. However, no systematic studies or reports on the role of AR in human oocytes have currently been performed or reported. This study aimed to explore the role of AR in human immature oocytes during in vitro maturation (IVM) and in vitro fertilization (IVF) in achieving better embryonic development and to provide a basis for the development of a pre-insemination culture medium specific for cumulus oocyte complexes (COCs).
UNASSIGNED: First, we examined the concentration of AR in the follicular fluid (FF) of patients who underwent routine IVF and explored the correlation between AR levels and oocyte maturation and subsequent embryonic development. Second, AR was added to the IVM medium to culture immature oocytes and investigate whether AR could improve the effects of IVM. Finally, we pioneered the use of a fertilization medium supplemented with AR for the pre-insemination culture of COCs to explore whether the involvement of AR can promote the maturation and fertilization of IVF oocytes, as well as subsequent embryonic development.
UNASSIGNED: A total of 609 FF samples were examined, and a positive correlation between AR levels and blastocyst formation was observed. In our IVM study, the development potential and IVM rate of immature oocytes, as well as the fertilization rate of IVM oocytes in the AR-added groups, were ameliorated significantly compared to the control group (All P < 0.05). Only the IVM-50 group had a significantly higher blastocyst formation rate than the control group (P < 0.05). In the final IVF study, the maturation, fertilization, high-quality embryo, blastocyst formation, and high-quality blastocyst rates of the AR-added group were significantly higher than those of the control group (All P < 0.05).
UNASSIGNED: AR levels in the FF positively correlated with blastocyst formation, and AR involvement in pre-insemination cultures of COCs can effectively improve laboratory outcomes in IVF. Furthermore, AR can directly promote the in vitro maturation and developmental potential of human immature oocytes at an optimal concentration of 50 ng/ml.