背景:乳腺癌对医生来说仍然是一个挑战。二甲双胍,一种抗糖尿病药物,显示有希望的抗癌特性对抗癌症。一种新兴的量子点(QD)材料可改善治疗剂的抗癌和成像性能。QD是纳米大小的颗粒,在纳米技术中具有极端的应用,被细胞捕获并积累在细胞内,提示生物成像和有效的抗癌结果。在这项研究中,一个简单的一锅水热法用于合成荧光二甲双胍衍生的碳点(M-CD),然后研究细胞毒性效应和对两种人乳腺癌细胞系的成像特征,包括,MCF-7和MDA-MB-231细胞。
结果:结果显示M-CD极大地降低了两种癌细胞的活力。IC50值显示M-CD在治疗后24-48小时比二甲双胍更具细胞毒性。癌细胞成功摄取M-CD,这导致细胞的形态变化和细胞内ROS水平的增加。在M-CD处理的细胞中,油红O阳性细胞的数量和caspase-3蛋白的表达增加。虚假因素包括,AMPK,mTOR,P62下调,而p-AMPK,Becline-1LC3I,和LC3II在M-CD处理的细胞中上调。最后,M-CD引起细胞的伤口愈合率降低。
结论:对于第一个,M-CD是通过简单的一锅水热处理合成的,无需进一步纯化。M-CD通过调节自噬信号抑制两种乳腺癌细胞。
BACKGROUND: Breast cancer remains a challenge for physicians. Metformin, an antidiabetic drug, show promising anticancer properties against cancers. An emerging quantum dot (QD) material improves therapeutic agents\' anticancer and imaging properties. QD are nano-sized particles with extreme application in nanotechnology captured by cells and accumulated inside cells, suggesting bioimaging and effective anticancer outcomes. In this study, a simple one-pot
hydrothermal method was used to synthesize fluorescent metformin-derived carbon dots (M-CDs) and then investigated the cytotoxic effects and imaging features on two human breast cancer cell lines including, MCF-7 and MDA-MB-231 cells.
RESULTS: Results showed that M-CDs profoundly decreased the viability of both cancer cells. IC50 values showed that M-CDs were more cytotoxic than metformin either 24-48 h post-treatment. Cancer cells uptake M-CDs successfully, which causes morphological changes in cells and increased levels of intracellular ROS. The number of Oil Red O-positive cells and the expression of caspase-3 protein were increased in M-CDs treated cells. Authophagic factors including, AMPK, mTOR, and P62 were down-regulated, while p-AMPK, Becline-1, LC3 I, and LC3 II were up-regulated in M-CDs treated cells. Finally, M-CDs caused a decrease in the wound healing rate of cells.
CONCLUSIONS: For the first, M-CDs were synthesized by simple one-pot
hydrothermal treatment without further purification. M-CDs inhibited both breast cancer cells through modulating autophagy signalling.