■本研究考察了2017年至2023年河南省人类偏肺病毒(HMPV)的季节性和遗传特征。
■对HMPV检测阳性的急性呼吸道感染(ARI)患者的样品进行实时逆转录聚合酶链反应。从这些样品中扩增G基因并测序以进行流行病学和系统发育分析。
■我们从2017年10月至2023年3月招募了2,707名ARI患者,发现HMPV阳性率为6.17%(167/2,707)。5岁以下儿童的感染率最高,为7.78%(138/1,774)。2018年和2019年HMPV疫情主要发生在春季(3月至5月),2018年5月的峰值阳性率为31.11%,2019年5月为19.57%。2020年11月出现了显著增长,当时的积极性达到了42.11%的历史高点,一直持续到2021年1月。从2021年2月到2023年3月,没有观察到明显的季节性高峰,率从0%到8.70%不等。在分析的81个G基因序列中,46.91%(38/81)被鉴定为A型亚型(A2c:45.67%,37/81;A2b:1.23%,1/81)和53.09%(43/81)为B型(B1:9.88%,8/81;B2:43.21%,35/81)。值得注意的是,在HMPV亚型中观察到AAABBA开关模式。优势菌株A亚型为A2c111nt-dup,B亚型为B2。
■河南省6年的监测详细介绍了HMPV的季节和遗传动态,为中国HMPV感染的控制和预防提供有价值的见解。这些发现支持靶向HMPV疫苗和免疫策略的开发。
UNASSIGNED: This study examines the seasonal and genetic characteristics of human metapneumovirus (HMPV) in Henan from 2017 to 2023.
UNASSIGNED: Samples from patients with acute respiratory infection (ARI) testing positive for HMPV were subjected to real-time reverse transcription polymerase chain reaction The G gene was amplified and sequenced from these samples for epidemiological and phylogenetic analysis.
UNASSIGNED: We enrolled 2,707 ARI patients from October 2017 to March 2023, finding an HMPV positivity rate of 6.17% (167/2,707). Children under five exhibited the highest infection rate at 7.78% (138/1,774). The 2018 and 2019 HMPV outbreaks predominantly occurred in spring (March to May), with peak positivity rates of 31.11% in May 2018 and 19.57% in May 2019. A notable increase occurred in November 2020, when positivity reached a historic high of 42.11%, continuing until January 2021. From February 2021 through March 2023, no significant seasonal peaks were observed, with rates ranging from 0% to 8.70%. Out of 81 G gene sequences analyzed, 46.91% (38/81) were identified as subtype A (A2c: 45.67%, 37/81; A2b: 1.23%, 1/81) and 53.09% (43/81) as subtype B (B1: 9.88%, 8/81; B2: 43.21%, 35/81). Notably, an AAABBA switch pattern was observed in HMPV subtypes. The dominant strains were A2c111nt-dup in subtype A and B2 in subtype B.
UNASSIGNED: Six years of surveillance in Henan Province has detailed the seasonal and genetic dynamics of HMPV, contributing valuable insights for the control and prevention of HMPV infections in China. These findings support the development of targeted HMPV vaccines and immunization strategies.