碘缺乏引起的甲状腺肿仍然是全球公共卫生问题,根据地理不同的表现形式,病人的年龄,和性爱。为了深入了解临床事件,一项回顾性研究分析了在Riehen社区医院接受手术的碘缺乏引起的甲状腺肿或甲状腺癌患者的病历,瑞士,1929年至1989年。尽管今天有足够的碘补充,在瑞士,碘依赖性甲状腺肿的风险仍然很大,表明遗传因素,其中,可能参与其中。因此,进行了一项试点研究,探索从这些病历中对血斑进行遗传分析的可行性,以调查和增强对甲状腺肿发展的理解,可能识别遗传变异,探讨饮食习惯和其他环境刺激对疾病的影响。每十年从医疗记录中收集甲状腺肿患者的器官扩大的血迹。这些指纹是通过按压制作的,绘图,或跟踪(即,将取出的器官压到纸片上。DNA分析显示,其产量之间的差异比年份之间的差异更大。相当大比例的样品表现出与样品收集时间和不同贡献者的DNA混合物无关的大量DNA降解。因此,每个甲状腺肿印记都必须单独评估,不能用于预测一般遗传分析的成功率。建议收集大量样本或整个血液消融以进行遗传分析,以减轻潜在的DNA数量不足。研究人员还应考虑降解和外部生物化合物对感兴趣的遗传分析的影响,预计主要贡献者来自患者的血液。
Iodine deficiency-induced
goiter continues to be a global public health concern, with varying manifestations based on geography, patient\'s age, and sex. To gain insights into clinical occurrences, a retrospective study analyzed medical records from patients with iodine deficiency-induced
goiter or thyroid cancer who underwent surgery at the Community Hospital in Riehen, Switzerland, between 1929 and 1989. Despite today\'s adequate iodine supplementation, a significant risk for iodine-independent
goiter remains in Switzerland, suggesting that genetic factors, among others, might be involved. Thus, a pilot study exploring the feasibility of genetic analysis of blood spots from these medical records was conducted to investigate and enhance the understanding of
goiter development, potentially identify genetic variations, and explore the influence of dietary habits and other environmental stimuli on the disease.Blood prints from goiter patients\' enlarged organs were collected per decade from medical records. These prints had been made by pressing, drawing, or tracing (i.e., pressed and drawn) the removed organs onto paper sheets. DNA analysis revealed that its yields varied more between the prints than between years. A considerable proportion of the samples exhibited substantial DNA degradation unrelated to sample collection time and DNA mixtures of different contributors. Thus, each
goiter imprint must be individually evaluated and cannot be used to predict the success rate of genetic analysis in general. Collecting a large sample or the entire blood ablation for genetic analysis is recommended to mitigate potential insufficient DNA quantities. Researchers should also consider degradation and external biological compounds\' impact on the genetic analysis of interest, with the dominant contributor anticipated to originate from the patient\'s blood.