对小叶Gnetum地上部分的植物化学研究导致分离出15种新的和8种已知结构多样的二苯乙烯。分离的化合物包含(E)-或(Z)-二苯乙烯(1-6,15-20),二氢二苯乙烯(21),苯基苯并呋喃(7,8,22),苄基化的二苯乙烯(9-11),苄基化的二苯乙烯二聚体(12),和含氮二苯乙烯(13a,13b,14)类型。新化合物的结构(1-12,13a,13b,14)是通过光谱分析以及实验和计算的ECD数据建立的。化合物12是通过苄基连接的第一二苯乙烯二聚体。在抗神经炎活性试验中,化合物4,5,9-11,13b,16-21对LPS诱导的BV-2小胶质细胞释放NO有明显的抑制作用,IC50值为0.35-16.1μM。化合物10具有最有效的活性(IC50=0.35μM),进一步的研究表明,它可以降低iNOS的mRNA水平,IL-1β,IL-6和TNF-α呈剂量依赖性。
Phytochemical investigation on the aerial parts of Gnetum parvifolium led to the isolation of 15 new and eight known structurally diverse stilbenes. The isolated compounds comprised (E)- or (Z)-stilbene (1-6, 15-20), dihydrostilbene (21), phenylbenzofuran (7, 8, 22), benzylated stilbene (9-11), benzylated stilbene dimer (12), and nitrogen-containing stilbene (13a, 13b, 14) types. The structures of the new compounds (1-12, 13a, 13b, 14) were established through spectroscopic analyses and experimental and calculated ECD data. Compound 12 is the first stilbene dimer connected through a benzyl group. In the anti-neuroinflammatory activity assay, compounds 4, 5, 9-11, 13b, and 16-21 displayed significant inhibitory effects against LPS-induced NO release in BV-2 microglial cells, with IC50 values of 0.35-16.1 μM. Compound 10 had the most potent activity (IC50 = 0.35 μM), and the further research indicated that it could decrease the mRNA levels of iNOS, IL-1β, IL-6, and TNF-α in a dose-dependent manner.