目的:调查患有活动性眼表疾病(OSD)的庇护猫的病原体流行情况。
方法:共有255只庇护所的家猫,有活跃的OSD证据。不正常,未受影响的猫被采样。
方法:对具有活性OSD的猫进行OSD评分。将合并的口咽/结膜拭子提交用于猫疱疹病毒(FHV-1)的RT-PCR/PCR,猫杯状病毒(FCV),衣原体属。(CHL),支气管败血波氏杆菌(BORD),和支原体属。(MYC)。
结果:病原体检测如下:76.4%(195/255)MYC,57.6%(147/255)FHV-1,42.7%(109/255)FCV,26.7%(68/255)CHL,和5.5%(14/255)的边界。单感染影响21.1%(54/255)的动物,MYC是最常见的单一感染(12.5%,32/255),其次是FHV-1(4.7%,12/255),其次是CHL(2.4%,6/255),其次是FCV(1.6%,4/255),没有动物有单一感染。双重感染影响了36.4%的动物(93/255),在30.1%(77/255)的双重感染中检测到MYC,在12.9%(33/255)的双重感染中检测到FCV。在9.8%(25/255)的动物中检测到MYC和FCV的双重感染。许多动物(35.3%,90/255)被发现受到3种或更多病原体的影响,和7.1%(18/255)的动物没有检测到病原体。OSD评分不受任何评估变量的影响,包括检测到的病原体的数量和类型。
结论:MYC,FHV-1、FCV、在这组OSD动物中通常检测到CHL。MYC和FCV(单独或彼此组合)在多个具有活跃OSD的动物中检测到,支持先前的证据,即任一者都可以独立地充当主要的眼表病原体。
OBJECTIVE: To survey the prevalence of pathogens in shelter-housed cats with active ocular surface disease (OSD).
METHODS: A total of 255 shelter-housed domestic cats with evidence of active OSD. No normal, unaffected cats were sampled.
METHODS: OSD scoring was performed on cats with active OSD. Combined oropharyngeal/conjunctival swabs were submitted for rt-PCR/PCR for feline herpesvirus (FHV-1), feline calicivirus (FCV), Chlamydia spp. (CHL), Bordetella bronchiseptica (BORD), and Mycoplasma spp. (MYC).
RESULTS: Pathogens were detected as follows: 76.4% (195/255) MYC, 57.6% (147/255) FHV-1, 42.7% (109/255) FCV, 26.7% (68/255) CHL, and 5.5% (14/255) BORD. Monoinfections affected 21.1% (54/255) animals, with MYC being the most common monoinfection (12.5%, 32/255), followed by FHV-1 (4.7%, 12/255), followed by CHL (2.4%, 6/255), followed by FCV (1.6%, 4/255), with no animals having a BORD monoinfection. Dual infections affected 36.4% of animals (93/255), with MYC detected in 30.1% (77/255) dual infections and FCV detected in 12.9% (33/255) dual infections. Dual infections with MYC and FCV together were detected in 9.8% (25/255) animals. Many animals (35.3%, 90/255) were found to be affected by 3 or more pathogens, and 7.1% (18/255) animals had no pathogens detected. OSD scores were not influenced by any variable assessed, including the number and type of pathogens detected.
CONCLUSIONS: MYC, FHV-1, FCV, and CHL were commonly detected in this group of animals with OSD. Both MYC and FCV (alone or in combination with each other) were detected in multiple animals with active OSD, supporting prior evidence that either may independently act as a primary ocular surface pathogen.