今天,塑料污染是对环境和公众健康的最大威胁之一。在暴露物种的组织中,微米和纳米碎片积累,导致遗传毒性,改变了新陈代谢,寿命缩短。研究纳米塑料(NPs)的基因毒性和促进肿瘤的潜力的模型是果蝇。在这里我们测试了聚苯乙烯,它通常用于食品包装,回收不好,至少占垃圾填埋场的30%。为了研究100μm聚苯乙烯纳米颗粒(PSNP)的生物学效应和致癌潜力,我们在受污染的食物上饲养了俄勒冈[R]野生型苍蝇。长时间暴露后,荧光PSNPs积累在肠道和脂肪体内。此外,以PSNP为食的果蝇显示出相当大的体重变化,发展时间,和寿命,以及从饥饿中恢复过来的能力受损。此外,我们注意到用PSNP喂养的DNAlig4突变体的运动活性降低,已知容易受到饮食压力的影响。对幼虫肠的qPCR分子研究显示,drice和p53基因的表达显着升高,表明对细胞损伤的反应。最后,我们使用疣缺陷突变体来评估PSNP的致癌潜力,并发现暴露的果蝇比未经治疗的果蝇有更多的异常质量。总之,我们的研究结果支持摄入纳米聚苯乙烯会触发暴露生物的代谢和遗传修饰的观点,最终延缓发育,加速死亡和疾病。
Today, plastic pollution is one of the biggest threats to the environment and public health. In the tissues of exposed species, micro- and nano-fragments accumulate, leading to genotoxicity, altered metabolism, and decreased lifespan. A model to investigate the genotoxic and tumor-promoting potential of nanoplastics (NPs) is Drosophila melanogaster. Here we tested polystyrene, which is commonly used in food packaging, is not well recycled, and makes up at least 30% of landfills. In order to investigate the biological effects and carcinogenic potential of 100 µm polystyrene nanoparticles (PSNPs), we raised Oregon [R] wild-type flies on contaminated food. After prolonged exposure, fluorescent PSNPs accumulated in the gut and fat bodies. Furthermore, PSNP-fed flies showed considerable alterations in weight, developmental time, and lifespan, as well as a compromised ability to recover from starvation. Additionally, we noticed a decrease in motor activity in DNAlig4 mutants fed with PSNPs, which are known to be susceptible to dietary stressors. A qPCR molecular investigation of the larval intestines revealed a markedly elevated expression of the genes drice and p53, suggesting a response to cell damage. Lastly, we used warts-defective mutants to assess the carcinogenic potential of PSNPs and discovered that exposed flies had more aberrant masses than untreated ones. In summary, our findings support the notion that ingested nanopolystyrene triggers metabolic and genetic modifications in the exposed organisms, eventually delaying development and accelerating death and disease.