Abstract：
Energy metabolism is essential for insect development, reproduction and detoxification. Insects often reallocate energy and resources to manage external stress, balancing the demands of detoxification and reproduction. Glucose transport 4 (Glut4), a glucose transporter, is involved in glucose and lipid metabolism. However, the specific molecular mechanism of Glut4 in insect reproduction, and its role in the response to insecticide-induced oxidative stress remain unclear. In this study, LmGlut4 was identified and analyzed in Locusta migratoria. Silencing of LmGlut4 significantly reduced vitellogenin (Vg) biosynthesis in the fat body and Vg absorption by oocytes, ultimately hindering ovarian development and oocyte maturation. Knockdown of LmGlut4 also inhibited the biosynthesis of key insect hormones, such as juvenile hormone (JH), 20-hydroxyecdysone (20E) and insulin. Furthermore, LmGlut4 knockdown led to reduced triglyceride (TG) and glycogen content in the fat body and ovary, as well as decreased capacity for trehalose biosynthesis in adipocytes. Additionally, dsLmGlut4-treated locusts showed heightened sensitivity to deltamethrin, leading to increased triglyceride depletion during detoxification. This study sheds light on the biological function of LmGlut4 in the ovary and provides potential target genes for exploring biological pest management strategies.
摘要：
能量代谢对昆虫的发育至关重要,繁殖和排毒。昆虫经常重新分配能量和资源来管理外部压力，平衡解毒和生殖的需求。葡萄糖转运4（Glut4），葡萄糖转运蛋白,参与葡萄糖和脂质代谢。然而,Glut4在昆虫繁殖中的特定分子机制，其在杀虫剂诱导的氧化应激反应中的作用尚不清楚。在这项研究中,LmGlut4在迁徙蝗虫中进行了鉴定和分析。沉默LmGlut4显著降低了脂肪体内的卵黄蛋白原(Vg)生物合成和卵母细胞对Vg的吸收,最终阻碍卵巢发育和卵母细胞成熟。敲除LmGlut4还抑制了关键昆虫激素的生物合成，例如幼体激素（JH），20-羟基蜕皮激素（20E）和胰岛素。此外，LmGlut4敲低导致脂肪体和卵巢中甘油三酯（TG）和糖原含量降低，以及脂肪细胞中海藻糖生物合成能力的降低。此外,dsLmGlut4处理的蝗虫对溴氰菊酯的敏感性提高，导致解毒过程中甘油三酯消耗增加。本研究揭示了LmGlut4在卵巢中的生物学功能，并为探索生物害虫管理策略提供了潜在的靶基因。
