内生真菌Xylariasp的代谢产物的研究。YM311647在固体发酵中分离出六种未描述的化合物,即木糖A-F,分别。其中包括一种对映烷倍半萜,三种愈创木烷倍半萜苷,和两种麦角烷醇苷.通过对光谱数据的广泛分析确定了化合物的结构,包括1D和2DNMR,以及HRESIMS数据。通过X-射线晶体学分析证实了苯二甲氧A的立体化学。测定了所有分离的化合物对7种植物病原真菌和2种人类病原真菌的抗真菌活性。其中,木糖A,E和F对测试的植物病原体显示出有效的活性。特别是,木杆菌氧化物E对赤霉素表现出最高的活性,月形弯孢菌,和炭疽菌,MIC值为4、4和8μg/mL,分别,与制霉菌素的阳性对照相当。有趣的是,很少有真菌来源的愈创烷倍半萜苷。此外,二甲苯氧化物E代表一种不寻常的天然存在的3,4-seco-甾体糖苷,在环A中带有七元内酯。
The investigation of the metabolites from the endophytic fungus Xylaria sp. YM 311647 in solid fermentation resulted in the isolation of six undescribed compounds, namely xylarioxides A-F, respectively. These included one
eremophilane sesquiterpene, three guaiane sesquiterpene glycosides, and two ergostane glycosides. The structures of the compounds were determined by extensive analyses of spectroscopic data, including 1D and 2D NMR, as well as HRESIMS data. The stereochemistry of xylarioxide A was confirmed by X-ray crystallographic analysis. All of the isolated compounds were assayed for their antifungal activities against seven phytopathogenic fungi and two human pathogenic fungi. Among them, xylarioxides A, E and F showed potent activities against the tested phytopathogens. Particularly, xylarioxide E exhibited the highest activity against Gibberella saubinetii, Curvularia lunata, and Colletotrichum gloeosporioides with MIC values of 4, 4, and 8 μg/mL, respectively, which were comparable to the positive control of nystatin. Interestingly, guaiane sesquiterpene glycosides have been rarely reported from fungal sources. Additionally, xylarioxide E represented an unusual naturally occurring 3,4-seco-steroidal glycoside with a seven-membered lactone in ring A.