■附睾精子保存是一种简单的保存方法,可以帮助防止农场动物的高遗传质量丧失。损失的机会增加,特别是在疾病爆发或其他正常生殖功能中断期间。
■这项研究调查了保存的公羊附睾精子使卵母细胞受精的能力。由于精子储存受精后的运动性成为一个问题,采用称为胞浆内精子注射方法的精子显微注射。
■研究分为两部分。首先,涉及附睾精子在5°C下保存12天。保存期间,精子质量参数,即运动性,生存能力,完整的膜,顶体,和脱氧核糖核酸(DNA)每三天评估一次。对于第二个实验中的生育力测试,成熟的卵母细胞被注射在保存的最后几天发现的不运动的精子。体外培养后原核发育的存在被用作精子激活和受精卵母细胞能力的指标。
■在保存时间内,所有精子质量参数均显着下降(p<0.05)。在第12天,发现运动性为0%,但是有活力的精子,精子膜完整,顶体,DNA保持在41.86%±9.30%,31.18%±5.15%,21.88%±1.93%,和33.35%±8.74%,分别。在生育能力测试中,我们从保存的第12天开始注射不活动的精子,发现的运动性最低,成熟的卵母细胞。这些精子能够激活(52.05%±7.15%)和受精(31.37%±1.75%)注射的卵母细胞,但与来自射精的精子相比,它们的受精能力显着降低(p<0.05)。
■在这项研究中,简单保存附睾精子会降低所有精子质量标准,特别是运动性。使用显微注射方法保存了没有运动性的精子,仍然证明了它激活和受精卵母细胞的能力。据此,这项研究提供了潜在的方法和工具,使用遗传优越的动物已经失去了执行定期受精的能力,也延长了生殖功能。
UNASSIGNED: Epididymal sperm preservation is a simple conservation approach that can help prevent the loss of high genetic quality of farm animals. The chance of loss increases, especially during disease outbreaks or other interruptions to normal reproduction function.
UNASSIGNED: This study looked into the ability of preserved ram epididymal sperm to fertilize oocytes. Due to motility becoming an issue following sperm storage for fertilization, the sperm microinjection known as intracytoplasmic sperm injection approach was employed.
UNASSIGNED: The study was divided into two parts. First, involved the preservation of epididymal sperm at 5°C for 12 days. During preservation, sperm quality parameters namely motility, viability, intact membrane, acrosome, and Deoxyribonucleic acid (DNA) are evaluated every three days. For the fertility test in the second experiment, matured oocytes were injected with immotile sperm discovered in the last days of preservation. The presence of pronucleus development following in vitro culture is used as an indicator of sperm\'s ability to activate and fertilize oocytes.
UNASSIGNED: All sperm quality parameters significantly (p < 0.05) declined during preservation time. On day 12, motility was discovered to be 0%, but viable sperm, sperm with intact membrane, acrosome, and DNA remained at 41.86% ± 9.30%, 31.18% ± 5.15%, 21.88% ± 1.93%, and 33.35% ± 8.74%, respectively. On the fertility test, we inject immotile sperm from day 12 of preservation, which has the lowest motility found, into matured oocytes. Those sperms are able to activate (52.05% ± 7.15%) and fertilize (31.37% ± 1.75%) the injected oocytes, but their fertilizing ability is significantly lower (p < 0.05) when compared to the sperm derived from the ejaculate.
UNASSIGNED: In this study, simple preservation of epididymal sperm reduces all sperm quality criteria, particularly motility. Using the microinjection approach preserved sperm which had no motility, still demonstrated its ability to activate and fertilize the oocytes. According to that, this study provides potential approaches and tools for using genetically superior animals that have lost their ability to execute regular fertilization, and also prolong reproduction function.