Abstract：
Aluminum phosphide (AlP) as an effective pesticide may contribute to oxidative stress and adversely influence sperm parameters. This study aimed to investigate the protective role of curcumin and nanocurcumin on oxidative damage in the testis of rats with AlP toxicity.
A total of 42 adult male Wistar rats were equally randomized into the following study groups (n = 7): Control, Control+Curcumin, Control+Nanocurcumin, AlP, AlP+Curcumin, and AlP+Nanocurcumin. The testis tissue was used to investigate the levels of testicular malondialdehyde (MDA), total oxidant status (TOS), total antioxidant capacity (TAC), and reduced glutathione (GSH) as well as the Catalase (CAT) and superoxide dismutase (SOD) enzyme activity. Epididymal sperm was used to perform sperm analysis.
AlP administration led to a significant increase in MDA, and TOS levels and also markedly decreased the SOD activity and the levels of TAC and GSH in testis tissue (p <0.001). Moreover, the motility and viability of sperms were significantly reduced (p <0.001). Curcumin and Nanocurcumin co-administration with AlP remarkably decreased the MDA and TOS level (p <0.001) and significantly increased the GSH and TAC levels as well as the activity of SOD in AlP intoxicated groups (p<0.001). Our findings demonstrated that Nanocurcumin administration has significantly enhanced the sperm quality in AlP intoxicated rats as compared to the control group (p <0.001).
According to the results of this study, Curcumin as a potential antioxidant could be an effective attenuative agent against AlP-induced oxidative damage in testis, especially when it is used in encapsulated form, nanocurcumin.
A total of 42 adult male Wistar rats were equally randomized into the following study groups (n = 7): Control, Control+Curcumin, Control+Nanocurcumin, AlP, AlP+Curcumin, and AlP+Nanocurcumin. The testis tissue was used to investigate the levels of testicular malondialdehyde (MDA), total oxidant status (TOS), total antioxidant capacity (TAC), and reduced glutathione (GSH) as well as the Catalase (CAT) and superoxide dismutase (SOD) enzyme activity. Epididymal sperm was used to perform sperm analysis.
AlP administration led to a significant increase in MDA, and TOS levels and also markedly decreased the SOD activity and the levels of TAC and GSH in testis tissue (p <0.001). Moreover, the motility and viability of sperms were significantly reduced (p <0.001). Curcumin and Nanocurcumin co-administration with AlP remarkably decreased the MDA and TOS level (p <0.001) and significantly increased the GSH and TAC levels as well as the activity of SOD in AlP intoxicated groups (p<0.001). Our findings demonstrated that Nanocurcumin administration has significantly enhanced the sperm quality in AlP intoxicated rats as compared to the control group (p <0.001).
According to the results of this study, Curcumin as a potential antioxidant could be an effective attenuative agent against AlP-induced oxidative damage in testis, especially when it is used in encapsulated form, nanocurcumin.
摘要：
目的：磷化铝（AlP）作为一种有效的农药可能会导致氧化应激并对精子参数产生不利影响。本研究旨在探讨姜黄素和纳米姜黄素对AlP毒性大鼠睾丸氧化损伤的保护作用。
方法：将42只成年雄性Wistar大鼠随机分为以下研究组（n=7）：对照组，控制+姜黄素，对照+纳米姜黄素，AlP,AlP+姜黄素,和AlP+纳米姜黄素。睾丸组织用于研究睾丸丙二醛（MDA）的水平，总氧化剂状态（TOS），总抗氧化能力(TAC)，和还原型谷胱甘肽（GSH）以及过氧化氢酶（CAT）和超氧化物歧化酶（SOD）酶活性。使用附睾精子进行精子分析。
结果：AlP给药导致MDA显著增加，和TOS水平，也显着降低了睾丸组织中SOD活性以及TAC和GSH的水平（p<0.001）。此外，精子活力和活力显著降低(p<0.001)。姜黄素和纳米姜黄素与AlP联合给药可显着降低MDA和TOS水平（p<0.001），并显着提高AlP中毒组的GSH和TAC水平以及SOD活性（p<0.001）。我们的发现表明，与对照组相比，纳米姜黄素的施用显着提高了AlP中毒大鼠的精子质量（p<0.001）。
结论：根据本研究的结果，姜黄素作为一种潜在的抗氧化剂可能是一种有效的抗AlP诱导的睾丸氧化损伤的衰减剂。特别是当它以封装形式使用时，纳米姜黄素.
方法：将42只成年雄性Wistar大鼠随机分为以下研究组（n=7）：对照组，控制+姜黄素，对照+纳米姜黄素，AlP,AlP+姜黄素,和AlP+纳米姜黄素。睾丸组织用于研究睾丸丙二醛（MDA）的水平，总氧化剂状态（TOS），总抗氧化能力(TAC)，和还原型谷胱甘肽（GSH）以及过氧化氢酶（CAT）和超氧化物歧化酶（SOD）酶活性。使用附睾精子进行精子分析。
结果：AlP给药导致MDA显著增加，和TOS水平，也显着降低了睾丸组织中SOD活性以及TAC和GSH的水平（p<0.001）。此外，精子活力和活力显著降低(p<0.001)。姜黄素和纳米姜黄素与AlP联合给药可显着降低MDA和TOS水平（p<0.001），并显着提高AlP中毒组的GSH和TAC水平以及SOD活性（p<0.001）。我们的发现表明，与对照组相比，纳米姜黄素的施用显着提高了AlP中毒大鼠的精子质量（p<0.001）。
结论：根据本研究的结果，姜黄素作为一种潜在的抗氧化剂可能是一种有效的抗AlP诱导的睾丸氧化损伤的衰减剂。特别是当它以封装形式使用时，纳米姜黄素.
