PDF(Pubmed)
Abstract：
The aim of this study was to evaluate the motility, morphology, and antioxidant status of European red deer sperm stored in a liquid state (variant I) and in the epididymides (variant II). Spermatozoa were harvested post-mortem from the cauda epididymis. Sperm samples in both variants were stored for up to six days (D6) at 5 °C. Spermatozoa were assessed for motility, viability, morphology, activity of antioxidant enzymes (superoxide dismutase, SOD; glutathione peroxidase, GPx; catalase, CAT), and lipid peroxidation (malondialdehyde, MDA, content). Sperm samples were analyzed on storage days 0, 2, 4, and 6 (D0-D6). Storage time and storage method significantly (p ≤ 0.05) influenced the examined variables. On D2, a decrease in motility and acrosomal integrity was observed in both storage variants, whereas a decrease in viability and an increase in MDA content were noted in spermatozoa stored in the epididymides. On D4, higher values of SOD and GPx activity and MDA content were noted in variant I than in variant II. Catalase activity was very low. GPx is the key enzyme that participates in the reduction of hydrogen peroxide in sperm cells. Spermatozoa stored in a liquid state were characterized by higher motility and viability, improved morphology and antioxidant status than those stored in the epididymides; therefore, liquid storage is more recommended for short-term preservation of epididymal spermatozoa.
摘要：
这项研究的目的是评估运动，形态学,以及以液态（变体I）和附睾（变体II）储存的欧洲马鹿精子的抗氧化状态。死后从附睾尾囊收获精子。两种变体中的精子样品在5°C下储存长达6天(D6)。评估精子的运动性，生存能力，形态学,抗氧化酶的活性(超氧化物歧化酶，SOD;谷胱甘肽过氧化物酶,GPx;过氧化氢酶,CAT),和脂质过氧化（丙二醛，MDA,内容)。在储存第0、2、4和6天(D0-D6)分析精子样品。储存时间和储存方法显着影响所检查的变量（p≤0.05）。在D2上，在两种储存变体中都观察到运动性和顶体完整性降低，而在附睾中储存的精子中，活力降低和MDA含量增加。在D4，变体I的SOD和GPx活性和MDA含量的值高于变体II。过氧化氢酶活性很低。GPx是参与精子细胞中过氧化氢还原的关键酶。以液态储存的精子具有较高的运动性和生存力，比储存在附睾中的形态和抗氧化状态得到改善；因此，液体储存更建议用于附睾精子的短期保存。
