这项研究的目的是描述不同形式的绿茶及其配方中的表儿茶素和黄酮醇的生物可及性和肠道吸收:散叶茶,茶粉,含GTE的35%儿茶素,和GTE与绿茶衍生的多糖和黄酮醇(CATEPLUS™)一起配制。通过使用Caco-2细胞的体外消化模型系统研究了表儿茶素和黄酮醇的生物可获得性和肠道吸收。散叶茶中总表儿茶素的生物可及性,茶粉,GTE,CATEPLUS™为1.27%,2.30%,22.05%,18.72%,分别,表明GTE和CATEPLUS™具有显著高于粉状茶和散叶茶的生物可及性。在粉末茶和散叶茶中均未检测到黄酮醇,但GTE和CATEPLUS™中总黄酮醇的生物可及性为85.74%和66.98%,分别。在CATEPLUS™(171.39±5.39ng/mg蛋白)中发现表儿茶素的肠道吸收最高,其次是GTE(57.38±9.31),茶粉(3.60±0.67),和散叶茶(2.94±1.03)。研究结果表明,将富含儿茶素的绿茶提取物与从绿茶加工中获得的第二成分一起配制可以提高表儿茶素的生物利用度。
The aim of this study was to profile the bioaccessibility and intestinal absorption of
epicatechins and flavonols in different forms of green tea and its formulation: loose leaf tea, powdered tea, 35% catechins containing GTE, and GTE formulated with green tea-derived polysaccharide and flavonols (CATEPLUS™). The bioaccessibillity and intestinal absorption of
epicatechins and flavonols was investigated by using an in vitro digestion model system with Caco-2 cells. The bioaccessibility of total
epicatechins in loose leaf tea, powdered tea, GTE, and CATEPLUS™ was 1.27%, 2.30%, 22.05%, and 18.72%, respectively, showing that GTE and CATEPLUS™ had significantly higher bioaccessibility than powdered tea and loose leaf tea. None of the flavonols were detected in powdered tea and loose leaf tea, but the bioaccessibility of the total flavonols in GTE and CATEPLUS™ was 85.74% and 66.98%, respectively. The highest intestinal absorption of
epicatechins was found in CATEPLUS™ (171.39 ± 5.39 ng/mg protein) followed by GTE (57.38 ± 9.31), powdered tea (3.60 ± 0.67), and loose leaf tea (2.94 ± 1.03). The results from the study suggest that formulating green tea extracts rich in catechins with second components obtained from green tea processing could enhance the bioavailability of
epicatechins.