慢性静脉疾病(CVD)是对静脉系统中一系列血液动力学变化的反应,这种疾病的发作通常是由妊娠引发的。胎盘组织对静脉高压中发生的特征性变化特别敏感。在这方面,细胞外基质(ECM)的变化,为了适应这种情况,是控制弹性生成的基础。因此,本研究的目的是分析诊断为CVD的女性胎盘绒毛中与弹性生成相关的蛋白质的mRNA和蛋白质表达水平的变化,在怀孕的第三个三个月。一个观察,进行了分析和前瞻性队列研究,其中将62名患有CVD的女性的胎盘与52名未诊断为CVD的女性的胎盘进行了比较。使用逆转录定量PCR和免疫组织化学分析基因和蛋白质表达水平。分别。结果表明,CVD妇女胎盘绒毛中EGFL7的基因和蛋白质表达水平显着降低。相比之下,ECM相关蛋白的基因和蛋白质表达水平显着增加,如原弹性蛋白,在患有CVD的女性的胎盘绒毛中也发现了腓骨蛋白4,原纤维蛋白1和赖氨酰氧化酶家族的成员(LOX和LOXL‑1)。据我们所知,本研究的结果首次表明,妊娠期间的CVD与胎盘绒毛EGFL7调节的弹性生成过程中必需成分的mRNA和蛋白质表达水平的变化有关.
Chronic venous disease (CVD) is the response to a series of hemodynamic changes in the venous system and the onset of this disease is often triggered by pregnancy. Placental tissue is particularly sensitive to the characteristic changes which occurs in venous hypertension. In this regard, changes in the extracellular matrix (ECM), that occur to adapt to this situation, are fundamental to controlling
elastogenesis. Therefore, the aim of the present study was to analyze the changes that occur in the mRNA and protein expression level of proteins related to
elastogenesis in the placental villi of women diagnosed with CVD, in the third trimester of pregnancy. An observational, analytical and prospective cohort study was conducted, in which the placenta from 62 women with CVD were compared with that in placenta from 52 women without a diagnosis of CVD. Gene and protein expression levels were analyzed using reverse transcription‑quantitative PCR and immunohistochemistry, respectively. The results showed a significant decrease in the gene and protein expression level of EGFL7 in the placental villi of women with CVD. By contrast, significant increases in the gene and protein expression level of ECM‑related proteins, such as tropoelastin, fibulin 4, fibrillin 1 and members of the lysyl oxidase family (LOX and LOXL‑1) were also found in the placental villi of women with CVD. To the best of our knowledge, the results from the present study showed for the first time that CVD during pregnancy was associated with changes in the mRNA and protein expression level in essential components of the EGFL7‑modulated
elastogenesis process in placental villi.