精油,从苗族药物Blumeabalsamifera(L.)DC。(BBO),是一种纯天然的伤口修复剂。它的应用有,然而,由于其低溶解度和高挥发性而受到限制。在这项研究中,我们开发了一种纳米乳液配方来改善BBO的特性。纳米乳液的粒径呈正态分布,其范围的71%集中在10-100nm之间,平均粒径为62.8nm,包封率为98%。申请7天后,BBO纳米乳(BBO-NE)组的伤口愈合率是正常BBO组的1.5倍。随着组织学观察,纳米乳液制剂已被证明显著提高BBO用于伤口修复的功效。此外,与BBO组相比,BBO-NE给药后炎症相关的TLR4、CD14和IRAK-1基因转录水平显著降低,下调幅度为47.8%,35.7%和57.8%,分别,而促炎因子IL-6和TNF-α的分泌也显著减少了83.8%和32.7%,分别,在纳米制剂给药(BBO-NE)组中与BBO组相比。相比之下,抗炎因子IL-10显著增加4.2倍。进一步发现,与BBO相比,在施用BBO-NE之后的不同时间点,每单位面积的药物渗透显著增加6.30%至19.5%。总之,纳米配方增强了BBO的药物渗透,减少炎症因子,抗炎因子水平升高,并促进胶原蛋白沉积,从而加速伤口修复。
The essential oil, extracted from the Hmong medicine Blumea balsamifera (L.) DC. (BBO), is a purely natural wound repair agent. Its application has, however, been restricted due to its low solubility and high volatility properties. In this study, we have developed a nanoemulsion formulation to improve the characteristics of BBO. The particle size of the nanoemulsion was normally distributed, and 71% of its range was concentrated between 10-100 nm, with an average particle size of 62.8 nm and an encapsulation rate of 98%. After 7 days of application, the wound healing rate of the BBO nanoemulsion (BBO-NE) group was 1.5 times higher than that of the normal BBO group. Along with histological observations, nanoemulsion formulation has been demonstrated to significantly improve the efficacy of BBO for wound repair. In addition, inflammation-related TLR4, CD14 and IRAK-1 gene transcript levels were significantly reduced after the administration of BBO-NE compared to the BBO group, with downregulation of 47.8%, 35.7% and 57.8%, respectively, while the secretion of pro-inflammatory factors IL-6 and TNF-α was also significantly reduced by 83.8% and 32.7%, respectively, in the nanoformulation administration (BBO-NE) group compared to the BBO group. In contrast, the anti-inflammatory factor IL-10 was significantly increased by 4.2-fold. It was further found that the drug penetration per unit area increased significantly 6.30% to 19.5% at different time points after the application of the BBO-NE compared to the BBO. In conclusion, nano-formulation enhanced the drug penetration of the BBO, reduced inflammatory factors, increased the level of anti-inflammatory factors, and promoted collagen deposition, thereby accelerating wound repair.