The objectives of this study were to explore the mechanism of stem mechanical strength in direct-seeded rice (DSR) as affected by paclobutrazol, especially its related endogenous hormone and cell wall component changes in culm tissue and response to the application of paclobutrazol. Field experiments were conducted in Changchun County, Jilin Province, China, by using two japonica rice varieties, Jiyujing and Jijing305, with soaking seeds in paclobutrazol at concentrations of (0 mg L-1, S0; 50 mg L-1; S1; 100 mg L-1; S2; 150 mg L-1, S3; 200 mg L-1, S4) in 2021 and 2022. The results suggest that the application of paclobutrazol increased the grain yield and reduced the lodging rate of DSR. Compared with the S0 treatments, soaking the seeds in paclobutrazol treatments rapidly shortened the length of the basal internode by decreasing the endogenous indole acetic acid (IAA) and gibberellin A3 (GA3) contents in culm tissue. The larger breaking strength (M) was attributed to a higher section modulus (SM) and bending stress (BS). The higher mechanical tissue thickness in culm tissue under paclobutrazol treatments, which was raised by higher endogenous zeatin and zeatin riboside (Z+ZR) content in culm tissue, increased the culm diameter, culm wall thickness, and section modulus (SM) of the internode. Compared with the S0 treatments, soaking the seeds in paclobutrazol treatments increased the cellulose content, lignin content, activities of lignin-related enzymes, and expression of key genes in lignin biosynthesis, as well as resulted in a higher bending stress (BS) to enhance the culm breaking strength (M).

Weed infestation is the major biological threat in direct-seeded rice production and can cause significant yield losses. The effective use of herbicides is particularly important in direct-seeded rice production. Anilofos, a pre-emergence herbicide, has been shown to be effective against the weed barnyardgrass. However, its impacts on crop yield and the direct-seeded rice production ecosystem remain underexplored. In this study, we conducted field trials and used untargeted metabolomics to investigate systemic effects of two different treatments (40 g/acre and 60 g/acre) on rice shoot and root as well as the rhizosphere soil during the critical tillering stage. Here, a total of 400 metabolites were determined in the crop and soil, with differential metabolites primarily comprising lipids and lipid-like molecules as well as phenylpropanoids and polyketides. Spearman correlation network analysis and a Zi-Pi plot revealed 7 key differential metabolites with significant topological roles, including succinic acid semialdehyde and riboflavin. KEGG pathway analysis showed that anilofos downregulated the amino acid metabolism while mainly promoted carbohydrate metabolism and secondary metabolites biosynthesis of the crop, which made minimal disruption on soil metabolism. Notably, we found 40 g/acre anilofos application could significantly improve the rice yield, potentially linked to the improved activity of flavonoid biosynthesis and starch and sucrose metabolism. This research provides a comprehensive evaluation of anilofos effects in the direct-seeded rice production system, offering new insights into optimizing herbicide use to improve agricultural sustainability and productivity.

BACKGROUND: Adjusting nitrogen (N) input based on actual seedling density (ASD) and plant N status is a practical approach for improving the yield stability of direct-seeded rice. However, the adjustment of topdressing N rates has been empirical in the past. This study aimed to establish a quantitative approach for determining N topdressing rates during tillering (Ntil ) and panicle development (NPI ) based on ASD and crop N status in direct-seeded rice. Field experiments were conducted involving 12 treatments, consisting of four Ntil and three seeding rates in 2017, and eight treatments combining seeding rate, Ntil , and NPI in 2020.
RESULTS: Linear regression analysis revealed that the tiller number at panicle initiation (TILPI ) was predominantly influenced by ASD and Ntil . The determination coefficients (R2 ) of the regression models ranged from 0.887 to 0.936 across the four-season experiments. The results indicated that Ntil could be determined accurately using ASD and the target maximum tiller number. Similarly, grain yield was influenced significantly by the N uptake at panicle initiation (NUPPI ) and NPI , with R2 of 0.814 and 0.783 in the early and late seasons of 2020, respectively. This suggested that NPI could be calculated based on NUPPI and the target grain yield.
CONCLUSIONS: The findings offer a quantitative method for establishing N topdressing rates for tillering and panicle development, relying on the monitoring of actual seedling density and plant N status in direct-seeded rice production. © 2023 Society of Chemical Industry.

Rice direct seeding technology has been considered as a promising alternative to traditional transplanting because of its advantages in saving labor and water. However, the poor emergence and seedling growth caused by chill stress are the main bottlenecks in wide-scale adoption of direct-seeded rice in Heilongjiang Province, China. Here, we found that natural plant growth regulator guvermectin (GV) effectively improved rice seed germination and seedling growth under chilling stress. Results from 2 year field trials showed that seed-soaking with GV not only enhanced the emergence rate and seedling growth but also increased the panicle number per plant and grain number per panicle, resulting in 9.0 and 6.8% increase in the yield of direct-seeded rice, respectively. Integrative physiological, transcriptomic, and metabolomic assays revealed that GV promoted seed germination under chilling stress mainly by enhancing the activities of α-amylase and antioxidant enzymes (superoxide dismutase, peroxidase, and catalase), increasing the contents of soluble sugar and soluble protein, improving the biosynthesis of glutathione and flavonoids, as well as activating gibberellin-responsive transcription factors and inhibiting the abscisic acid signaling pathway. These findings indicate that seed-soaking with GV has good potential to improve seedling establishment and yield of direct-seeded rice even under chilling stress.

BACKGROUND: Enhancing grain yield and nitrogen use efficiency (NUE) of rice is of great importance for sustainable agricultural development. Little effort has been made to increase grain yield and NUE of direct-seeded rice under the double-cropping system in South China. Field trials were conducted during 2018-2020 with four treatments, including nitrogen-free, farmers\' fertilization practice (FP), \'three controls\' nutrient management (TC), and simplified and nitrogen-reduced practice (SNRP).
RESULTS: Grain yield under SNRP averaged 6.46 t ha-1 during the three years and was 23.0% higher than that of FP but comparable to that of TC. Recovery efficiency (REN ), agronomic efficiency (AEN ), and partial factor productivity (PFPN ) of nitrogen under SNRP increased by 12.0-22.7%, 159.3-295.0% and 94.6-112.5% respectively compared with FP. Harvest index and sink capacity increased by 7.3-10.8% and 14.9-21.3% respectively. Percentage of productive tillers (PPT) and biomass after heading increased by 24.0% and 104.5% respectively. Leaf nitrogen concentration at heading and nitrogen accumulation after heading increased by 16.3% and 842.0% respectively. Grain yield was positively correlated with PPT, sink capacity, harvest index, biomass and nitrogen accumulation after heading, REN , AEN , and PFPN .
CONCLUSIONS: Grain yield and NUE under SNRP were superior to those under FP and comparable to those under TC. Increase in sink capacity, higher PPT, more biomass and nitrogen accumulation after heading, and greater harvest index were responsible for high grain yield and NUE in SNRP with reduced nitrogen fertilizer and labor input. SNRP is a feasible approach for direct-seeded rice under a double-cropping system in South China. © 2023 Society of Chemical Industry.

Paddy fields are complex ecosystems that both emit CH4 and absorb CO2, which plays an important role in the global water-carbon cycle and carbon budget. In this study, the CH4 fluxes and CO2 fluxes of double-cropping direct-seeded rice fields in 2020 in the Poyang Lake Plain were obtained using the eddy covariance method, and the variation characteristics, accumulation in the whole growth period, and comprehensive greenhouse effects of two greenhouse gases were quantitatively revealed. The results showed that, the double-cropping direct-seeded rice field in Poyang Lake Plain was the source of CH4 emission, and the emission during the whole growth period was 52.6 g·m-2, with an average daily emission of 0.208 g·(m2·d)-1. CH4 emission and daily average emission in the early rice season were 20.7 g·m-2 and 0.188 g·(m2·d)-1, respectively, which were lower than the emissions of 31.9 g·m-2 and 0.255 g·(m2·d)-1 in the late rice season. CH4 flux had significant seasonal variation characteristics. The strong emission period (emission peak) of CH4 was concentrated in the middle growth stage of early rice and the early growth stage of late rice. A total of 85.5% of CH4 in the early rice season and 92.1% of CH4 in the late rice season were released during the strong emission periods, and seasonal peak values were 0.638 g·(m2·d)-1 and 1.282 g·(m2·d)-1, respectively. The diurnal variation characteristics of CH4 flux showed three types:obvious unimodal type, non-obvious unimodal type, and irregular type. The strong emission period was mainly the unimodal type, and the peak values of 0.453 μmol·(m2·s)-1 in the early rice season and 0.977 μmol·(m2·s)-1 in the late rice season appeared at 14:00-15:00 and maintained a high emission rate at 12:30-16:00. The CO2 accumulation in the whole growth period of early rice and late rice was -990.4 g·m-2 and -1156.6 g·m-2, respectively, and the total was -2147.0 g·m-2. The comprehensive greenhouse effect of CH4 emission and CO2 exchange in the double-cropping paddy field was -673.6 g·m-2 (calculated using the CO2 equivalent), which showed a cooling effect. Excluding CH4 emissions when evaluating the greenhouse effect of the paddy field, the CO2 equivalent emission of 1473.4 g·m-2 would be underestimated, accounting for 68.6% of the net CO2 absorption. Considering CH4 emissions, CO2 exchanges, and carbon emissions caused by rice harvest, the two-season direct seeding paddy field in Poyang Lake Plain was the source of greenhouse gas emissions.

BACKGROUND: Direct-seeded rice has been developed rapidly because of labor savings. Changes in rice cultivation methods put forward new requirements for nitrogen (N) fertilizer management practices. Field experiments with five different fertilizer ratios of basal, tillering and panicle fertilizer, namely N1 (10:0:0), N2 (6:2:2), N3 (4:3:3), N4 (2:4:4) and N5 (0:5:5), were conducted to investigate the effects of different N fertilizer management practices on yield formation, N uptakes, and ammonia (NH3 ) volatilization from paddy fields in direct-seeded rice.
RESULTS: The results showed that the N4 treatment improved grain yield by 5.1% while decreasing NH3 volatilization by 20.4% compared with that of conventional fertilizer treatment (N2). The panicle number per unit area was the key factor to determine the yield of direct-seeded rice (72%). Excessive N application of basal fertilizer (N1) reduced seedling emergence, N use efficiency, and yield by 45.3%, 160.6%, and 6.9% respectively and increased NH3 volatilization by 28.1% compared with that of the N4 treatment. Removal of basal N fertilizer (N5) N reduced spike number and yield by 13.0% and 6.9% respectively, minimizing NH3 volatilization while affecting the construction of high-yielding populations compared with that of the N4 treatment.
CONCLUSIONS: Optimized N fertilizer management achieved delayed senescence (maintenance of higher leaf Soil Plant Analysis Development meter values in late reproduction), higher canopy photoassimilation (suitable leaf area), higher N fertilizer use efficiency, and less N loss (lower cumulative NH3 volatilization). © 2023 Society of Chemical Industry.

Weeds are one of the key threats in sustaining the productivity of the rice-wheat cropping system in the Indo-Gangetic Plains. The development of sound integrated weed management technologies requires knowledge of mechanisms that influence weed flora composition and weed seedbank dynamics. A long-term study was initiated in 2015 at Patna, Bihar, India to evaluate the effect of seven tillage and crop establishment methods on weed density, weed seedbank composition, and crop productivity in rice-wheat-mungbean rotation. All the treatments included zero-till mungbean after wheat. Tillage and crop establishment methods had differential effects on weed and weed seedbank composition. In rice, zero-till direct-seeded rice recorded 62% lower emergence of Cyperus iria, 82-90% of Echinochloa colona, and 81-83% of total weeds compared to tilled systems, but the system of rice and wheat intensification favoured E. colona. In wheat, the system of wheat intensification favoured the Phalaris minor and Solanum nigrum. Zero-till rice and wheat reduced the seedbank of Trianthema portulacastrum by 95%, and total weed seedbank by 62% compared to the system of rice and wheat intensification. Nearly, 72% of C. iria seeds, 62% of grasses, and 64% of broad-leaved weeds were in 0-15 cm soil layer. Zero-till direct-seeded rice produced a 13% lower rice grain yield than conventional puddled transplanted rice. Compared to the system of wheat intensification, zero-till wheat under triple zero-till systems produced an 11.5% higher grain yield. Managing weed seedbank is a long-term endeavour. The present study revealed that tillage and crop establishment methods influence weed density and diversity. Under zero-till rice-wheat system, rice yield decreases marginally, but the system productivity maintains due to improvement in succeeding wheat yield. This system is also helpful in reducing the weed flora density and soil weed seedbank. Regular monitoring and management of emerging pests such as armyworm (Mythimna separata) are, however, required. The study suggests that the adoption of triple zero-tillage can be a viable option for reducing the weed density and weed seedbank concurrently increasing the system productivity of the rice-wheat-mungbean cropping system in eastern Indo-Gangetic Plains.

Rice production has increased significantly with the efforts of international research centers and national governments in the past five decades. Nonetheless, productivity improvement still needs to accelerate in the coming years to feed the growing population that depends on rice for calories and nutrients. This challenge is compounded by the increasing scarcity of natural resources such as water and farmland. This article reviews 17 ex-post impact assessment studies published from 2016 to 2021 on rice varieties, agronomic practices, institutional arrangements, information and communication technologies, and post-harvest technologies used by rice farmers. From the review of these selected studies, we found that stress-tolerant varieties in Asia and Africa significantly increased rice yield and income. Additionally, institutional innovations, training, and natural resource management practices, such as direct-seeded rice, rodent control, and iron-toxicity removal, have had a considerable positive effect on smallholder rice farmers\' economic well-being (income and rice yield). Additional positive impacts are expected from the important uptake of stress-tolerant varieties documented in several Asian, Latin American, and African countries.

Chilling stress is the major abiotic stress that severely limited the seedling establishment of direct-seeded rice in temperate and sub-tropical rice production regions. While seed priming is an efficient pre-sowing seed treatment in enhancing crop establishment under abiotic stress. Our previous research has identified two seed priming treatments, selenium priming (Se) and salicylic priming (SA) that effectively improved the seed germination and seedling growth of rice under chilling stress. To further explore how seed priming enhance the starch degradation of rice seeds under chilling stress, the present study evaluated the effects of Se and SA priming on germination and seedling growth, α-amylase activity, total soluble sugar content, hormone content and associated gene relative expression under chilling stress. The results showed that both Se and SA priming significantly increased the seed germination and seedling growth attributes, and enhanced the starch degradation ability by increasing α-amylase activity and total soluble sugar content under chilling stress. Meanwhile, seed priming increased the transcription level of OsRamy1A, OsRamy3B that regulated by GA, and increased the transcription level of OsRamy3E that regulated by sugar signals. Furthermore, seed priming significantly improved the GA3 contents in rice seeds by up-regulating the expression of OsGA3ox1 and OsGA20ox1, and decreased the ABA content and the expression of OsNCED1, indicating that the improved starch degradation ability in primed rice seeds under chilling stress might be attributed to the increased GA3 and decreased ABA levels in primed rice seeds, which induced the expression of GA-mediated α-amylase. However, studies to explore how seed priming mediate hormonal metabolism and the expression of OsRamy3E are desperately needed.