海马子场前丘(ProS),是小鼠的一个保守的神经解剖区域,猴子,和人类。该区域位于CA1和下体(Sub)之间,特别是在其边界上缺乏共识;报告对其特征和位置的描述各不相同。在这份报告中,我们回顾,精炼,并评估将ProS与其相邻子场区分开的四个细胞结构特征:(1)小神经元,(2)轻度染色的神经元,(3)浅层簇状神经元,和(4)小区稀疏区。在所有情况下都描述了ProS(n=10)。检查了ProS的细胞结构特征和尾部位置,从头部前部穿过海马体。最常见的特征是小的锥体神经元,在ProS中与较大的锥体神经元混合在一起。我们定量测量了ProS锥体神经元,显示(平均值,锥体底部宽度=14.31µm,n=每个子场400)。CA1神经元平均15.57µm,亚神经元平均15.63µm,两者都与ProS显著不同(Kruskal-Wallis检验,p<.0001)。观察到的其他三个特征是轻度染色的神经元,成簇的神经元,和细胞稀疏区。一起来看,这些发现表明ProS是一个独立的子领域,可能对更广泛的相互连接的海马网络有不同的功能贡献。我们的结果表明,ProS是一个细胞结构变化的子领域,无论是特征还是个人之间。ProS的功能和个人的这种多样化体系结构可以解释关于该子字段识别的长期复杂性。
The hippocampal subfield prosubiculum (ProS), is a conserved neuroanatomic region in mouse, monkey, and human. This area lies between CA1 and subiculum (Sub) and particularly lacks consensus on its boundaries; reports have varied on the description of its features and location. In this report, we review, refine, and evaluate four cytoarchitectural features that differentiate ProS from its neighboring subfields: (1) small neurons, (2) lightly stained neurons, (3) superficial clustered neurons, and (4) a cell sparse zone. ProS was delineated in all cases (n = 10). ProS was examined for its cytoarchitectonic features and location rostrocaudally, from the anterior head through the body in the hippocampus. The most common feature was small pyramidal neurons, which were intermingled with larger pyramidal neurons in ProS. We quantitatively measured ProS pyramidal neurons, which showed (average, width at pyramidal base = 14.31 µm, n = 400 per subfield). CA1 neurons averaged 15.57 µm and Sub neurons averaged 15.63 µm, both were significantly different than ProS (Kruskal-Wallis test, p < .0001). The other three features observed were lightly stained neurons, clustered neurons, and a cell sparse zone. Taken together, these findings suggest that ProS is an independent subfield, likely with distinct functional contributions to the broader interconnected hippocampal network. Our results suggest that ProS is a cytoarchitecturally varied subfield, both for features and among individuals. This diverse architecture in features and individuals for ProS could explain the long-standing complexity regarding the identification of this subfield.