PDF(Pubmed)
Abstract：
Cytoarchitecture, the organization of cells within organs and tissues, serves as a crucial anatomical foundation for the delineation of various regions. It enables the segmentation of the cortex into distinct areas with unique structural and functional characteristics. While traditional 2D atlases have focused on cytoarchitectonic mapping of cortical regions through individual sections, the intricate cortical gyri and sulci demands a 3D perspective for unambiguous interpretation. In this study, we employed fluorescent micro-optical sectioning tomography to acquire architectural datasets of the entire macaque brain at a resolution of 0.65 μm × 0.65 μm × 3 μm. With these volumetric data, the cortical laminar textures were remarkably presented in appropriate view planes. Additionally, we established a stereo coordinate system to represent the cytoarchitectonic information as surface-based tomograms. Utilizing these cytoarchitectonic features, we were able to three-dimensionally parcel the macaque cortex into multiple regions exhibiting contrasting architectural patterns. The whole-brain analysis was also conducted on mice that clearly revealed the presence of barrel cortex and reflected biological reasonability of this method. Leveraging these high-resolution continuous datasets, our method offers a robust tool for exploring the organizational logic and pathological mechanisms of the brain\'s 3D anatomical structure.
摘要：
细胞建筑学，器官和组织内的细胞组织，作为描绘各个区域的关键解剖学基础。它可以将皮质分割成具有独特结构和功能特征的不同区域。虽然传统的2D图集专注于通过单个切片对皮质区域进行细胞结构映射，复杂的皮质回旋和沟需要3D视角进行明确的解释。在这项研究中,我们使用荧光显微光学切片层析成像技术以0.65μm×0.65μm×3μm的分辨率获取整个猕猴大脑的建筑数据集。有了这些体积数据，皮质层状纹理在适当的视图平面中得到了显着呈现。此外,我们建立了一个立体坐标系来将细胞结构信息表示为基于表面的断层图像。利用这些细胞结构特征，我们能够将猕猴皮层三维地分成多个区域，这些区域表现出对比鲜明的建筑模式。还对小鼠进行了全脑分析，清楚地揭示了桶状皮质的存在并反映了该方法的生物学合理性。利用这些高分辨率连续数据集，我们的方法为探索大脑3D解剖结构的组织逻辑和病理机制提供了一个强大的工具。
