微藻小球藻用于处理酿酒厂废水(WWW)。最初进行了批量实验,以研究如何在不同的培养基中适应生物质,废水稀释,氨氮(NH4-N)的添加影响微藻的生长和主要污染物的去除。之后,两个序批式反应器(SBR)系统进行了测试,应用不同的配置和水力停留时间。在实验结束时收集的生物质被表征为蛋白质,脂质,碳水化合物,氨基酸谱,还有叶黄素的存在,β-胡萝卜素,叶绿素a,和生育酚。批量实验表明,小球藻对城市废水的适应增强了NH4-N和总磷(TP)的去除。两级SBR系统的运行实现了COD和NH4-N去除率分别为85±9%和91±20%,分别,而使用单级系统给料厌氧预处理的WWW导致COD和NH4-N去除率为78±9%和95±9%,分别。在添加NH4-N的分批实验以及SBR实验中,对生物质的分析显示出更高的蛋白质含量(高达58.8%)。在SBR条件下培养微藻可提高色素和生育酚的产量。叶黄素的最大浓度为1075mgkg-1,45.5mgkg-1和131.2mgkg-1,β-胡萝卜素,和生育酚,分别,在一个阶段的系统。我们的研究结果表明,在WWW中种植小球藻不仅可以从WWW中去除营养,而且还可能用于食品工业中使用的增值成分的生产。化妆品,和动物饲料。
The microalgae Chlorella sorokiniana was used for the treatment of winery wastewater (WWW). Batch experiments were initially conducted to investigate how biomass acclimatization in different media, dilution of wastewater, and addition of ammonium nitrogen (NH4-N) affect the growth of microalgae and the removal of major pollutants. Afterwards, two sequencing batch reactor (SBR) systems were tested applying different configurations and hydraulic retention times. The biomass collected at the end of the experiments was characterized for proteins, lipids, carbohydrates, amino acid profile, and the existence of lutein, β-carotene, chlorophyll a, and tocopherols. Batch experiments showed that Chlorella sorokiniana acclimatization to urban wastewater enhanced the removal of NH4-N and total phosphorus (TP). The operation of a two-stage SBR system achieved COD and NH4-N removal equal to 85 ± 9% and 91 ± 20%, respectively, while the use of a single-stage system feeding with anaerobically pretreated WWW resulted to COD and NH4-N removal of 78 ± 9% and 95 ± 9%, respectively. Analyses of biomass showed higher protein content (up to 58.8%) in batch experiments with NH4-N addition as well as in SBR experiments. The
cultivation of microalgae under SBR conditions enhanced the production of pigments and tocopherols. The maximum concentrations of 1075 mg kg-1, 45.5 mg kg-1, and 131.2 mg kg-1 were achieved for lutein, β-carotene, and tocopherols, respectively, in the one-stage system. Our findings suggested that Chlorella sorokiniana
cultivation in WWW not only removed nutrients from WWW but also could potentially serve for the production of value-added ingredients used in food industry, cosmetics, and animal feedstock.