Abstract：
Earth harbors unique environments where only microorganisms adapted to extreme conditions, known as extremophiles, can survive. This study focused on a high-altitude meltwater pond, located in the Puna de Atacama, Dry Andes. The extremophilic bacteria of this habitat must adapt to a range of extremities, including cold and dry climate, high UV radiation, high daily temperature fluctuations, low-nutrient availability, and negative water balance. This study aimed to explore the taxonomic diversity of cultivable extremophilic bacteria from sediment samples of a desiccated, high-altitude, meltwater pond using media with different organic matter contents and different incubation temperatures. Based on the 16S rRNA gene sequence analysis, the isolates were identified as members of the phyla Actinobacteria, Proteobacteria, and Firmicutes. The most abundant genera were Arthrobacter and Pseudoarthrobacter. The isolates had oligocarbophilic and psychrotrophic properties, suggesting that they have adapted to the extreme environmental parameters of their natural habitats. The results indicate a positive correlation between nutrient concentration and temperature tolerance.
摘要：
地球拥有独特的环境，只有微生物才能适应极端条件，被称为极端微生物，可以生存。这项研究集中在一个高海拔的融化水塘，位于阿塔卡马普纳，干燥的安第斯山脉。这个栖息地的极端细菌必须适应一系列的四肢，包括寒冷和干燥的气候，高紫外线辐射，高温每日波动，养分利用率低，和负水平衡。本研究旨在探索干燥沉积物样品中可培养的嗜极端细菌的分类学多样性，高海拔,使用不同有机物含量和不同孵育温度的培养基的熔融水塘。基于16SrRNA基因序列分析,分离物被鉴定为门放线菌的成员，变形杆菌,和Firmicutes。最丰富的属是节杆菌和假节杆菌。这些分离物具有低聚嗜碳和嗜冷特性，表明它们已经适应了自然栖息地的极端环境参数。结果表明，养分浓度与温度耐受性呈正相关。
