常见的marmosets(Callithrixjacchus;CMs)是广泛用于生物医学研究的小型新世界灵长类动物。此类研究的早期阶段通常包括体外实验,其需要来自不同组织的标准化和充分表征的CM细胞培养物。尽管CMs的实验室工作历史悠久,并且此类研究具有很高的翻译潜力,可用标准化的数量,定义明确,稳定,和验证的CM细胞系仍然很小。虽然原代细胞和永生化细胞系主要用于感染性疾病的研究,生化研究,靶向基因治疗,目前CM胚胎干细胞和诱导多能干细胞的主要应用是再生医学,干细胞研究,转基因CM的产生,移植学,细胞疗法,生殖生理学,肿瘤学,和神经退行性疾病。在这篇综述中,我们总结了关于主要优势的数据,迄今为止发表的CM细胞系的缺点和研究应用,包括原代细胞,永生化细胞系,淋巴母细胞细胞系,胚胎干细胞,和诱导多能干细胞。
Common marmosets (Callithrix jacchus; CMs) are small New World primates widely used in biomedical research. Early stages of such research often include in vitro experiments which require standardized and well-characterized CM cell cultures derived from different tissues. Despite the long history of laboratory work with CMs and high translational potential of such studies, the number of available standardized, well-defined, stable, and validated CM cell lines is still small. While primary cells and immortalized cell lines are mostly used for the studies of infectious diseases, biochemical research, and targeted gene therapy, the main current applications of CM embryonic stem cells and induced pluripotent stem cells are regenerative medicine, stem cell research, generation of transgenic CMs, transplantology, cell therapy, reproductive physiology, oncology, and neurodegenerative diseases. In this review we summarize the data on the main advantages, drawbacks and research applications of CM cell lines published to date including primary cells, immortalized cell lines, lymphoblastoid cell lines, embryonic stem cells, and induced pluripotent stem cells.