代谢综合征(MetS)的数量正在增加,和鱼磷脂饮食可以降低MetS的风险。在这项研究中,通过广泛的脂质组学分析鱼磷脂饮食对代谢综合征小鼠结肠内容物的脂质代谢变化,通过建立MetS模型将小鼠随机分为不同饮食类型的实验组。14周后,处死小鼠并收集血清和结肠内容物。超高液相串联质谱用于广泛靶向脂质组学分析,定性定量检测小鼠结肠内容物脂代谢变化。在鱼类磷脂的干预下,MetS小鼠被显著抑制,血清总胆固醇(TC)和甘油三酯(TG)降低,血清高密度脂蛋白(HDL-C)和低密度脂蛋白(LDL-C)水平均有改善,空腹血糖和胰岛素水平下降,炎症因子降低。通过筛选,研究发现,33种脂质代谢产物可能是关键代谢产物,其中5种代谢途径发生了显著变化。模块化脂质代谢物,有可能了解不同类型和浓度的鱼磷脂对代谢综合征的影响程度。因此,我们的研究可能为改善MetS提供新的治疗线索.
The number of metabolic syndromes (MetS) is increasing, and a fish phospholipid diet can reduce the risk of MetS. In this study, the changes in lipid metabolism of colon contents were analyzed by extensive lipidomics in mice with metabolic syndrome by fish phospholipid diet, and mice were randomly divided into experimental groups with different diet types by establishing a MetS model. After 14 weeks, the mice were sacrificed and the serum and colon contents were collected. Ultra-high liquid phase tandem mass spectrometry was used for broadly targeted lipidomic analysis, and the qualitative and quantitative detection of lipid metabolism changes in the colonic contents of mice. Under the intervention of fish phospholipids, MetS mice were significantly inhibited, serum total cholesterol (TC) and triglycerides (TG) decreased, serum high-density lipoprotein (HDL-C) and low-density lipoprotein (LDL-C) levels were improved, fasting blood glucose and insulin levels decreased, and inflammatory factors decreased. Through screening, it was found that thirty-three lipid metabolites may be key metabolites and five have significantly changed metabolic pathways. Modularizing lipid metabolites, it is possible to understand the extent to which different types and concentrations of fish phospholipids affect metabolic syndrome. Therefore, our study may provide new therapeutic clues for improving MetS.