背景:双酚A(BPA)是一种广泛使用的化学物质,对动物和人类具有有害作用。新生儿和幼年期是一个高风险的神经发育期。
目的:本研究旨在确定给予母亲低剂量BPA对雄性白化病幼鼠大脑皮层的改变以及氧化应激的作用。
方法:将30只孕鼠随机分成3组,阴性对照,和阳性对照:每天一次通过胃管接受1cc玉米油,并处理BPA:剂量为200μg/kg/天(溶解在1cc玉米油中)。各组雄性幼鼠在1周时处死,3周和6周。然后将大脑与大脑分离以进行组织学和生化研究。
结果:服用BPA的大鼠脂质过氧化标志物(MDA)水平升高,酶促抗氧化剂(SOD和CAT)水平降低,大脑重量,和降低非酶抗氧化防御(GSH)的水平。从病理上讲,锥体细胞萎缩,血管充血。与对照组相比,GFAP显示出阳性免疫反应性星形胶质细胞的数量增加,BPA治疗组的面积百分比增加具有统计学意义。与MBP相反。半薄和超薄BPA切片显示有髓鞘的轴突发生退行性变化,核小核和核膜破裂。溶酶体,内质网池扩张,无髓神经纤维明显增加。
结论:由于氧化应激,BPA对发育中的大脑皮层的结构产生负面影响。
BACKGROUND: Bisphenol A (BPA) is a widely used chemical with a harmful effect on animal and human. The neonatal and juvenile period is a highly risky neurodevelopmental period.
OBJECTIVE: This study aimed to determine how male albino rat pups\' cerebral cortex was altered by low doses of BPA given to mothers and the role of the oxidative stress.
METHODS: Thirty pregnant rats were randomly split into three equal groups, negative control, and positive control: received 1 cc of corn oil once a day through gastric tube and BPA treated: a dose of 200 µg/kg/day (dissolved in 1 cc corn oil). The male rat pups of each group were sacrificed at 1 week, 3 weeks and 6 weeks. The cerebra were then separated from the brain for histological and biochemical studies.
RESULTS: Rats administered BPA had raised levels of lipid peroxidation marker (MDA), lower levels of enzymatic antioxidants (SOD and CAT) with decreased body, cerebral weights, and decreased levels of non-enzymatic antioxidant defense (GSH). Histo-pathologically, shrunken pyramidal cells with congested blood vessels appeared. GFAP displayed increased number of positive immune-reactive astrocytes with high statistically significant increase in the area % in BPA treated group when compared to the control groups, on contrary to MBP. Semi-thin and ultra-thin BPA-sections revealed degenerative changes in myelinated axons with tiny nucleus and broken nuclear membranes. Lysosomes, dilated endoplasmic reticulum cisternae with noticeable increase in unmyelinated nerve fibers were also observed.
CONCLUSIONS: The structure of the developing cerebral cortex is negatively impacted by BPA due to oxidative stress.