■放射摄影术是综合牙科护理中使用的最有价值的诊断工具之一。牙科X光片的辐射被认为会引起细胞遗传学变化,其合理的影响可能会持续几个小时,月,或几代人,尤其是儿童。
■评估和比较常规使用的口腔根尖周放射学暴露和放射学暴露对脱落上皮细胞的可能的遗传毒性作用,该作用是通过在单次访问牙髓切除术过程中使用口腔微核细胞(BMCyt)测定进行测量的。
■研究包括60名健康儿童,他们在单次就诊牙髓切除术的各个步骤中接受了口内心尖造影(IOPAR;第1组,n=30)或放射造影(RVG;第2组,n=30)。在X射线暴露之前和暴露后10±2天从颊粘膜取细胞学涂片。用Feulgen对细胞进行染色,并通过对每个样品1,000个细胞进行评分来评估微核。
■来自口腔内根尖周X线照相术的辐射暴露的基因毒性作用高于RVG,表明微核(MN)形成显着增加。
■在IOPAR或RVG期间发射的X射线辐射确实以微核频率增加的形式引起基因毒性变化。所以,如有必要,应非常小心并遵循标准方案,以建议进行X射线照相,并减少辐射暴露的累积生物学效应。
■考虑到DNA损伤与癌变之间关系的有力证据以及口腔内X光片在儿科牙科中的广泛应用,了解这些牙科X射线在多大程度上引起基因毒性作用,从而对口腔粘膜造成DNA损伤,这将是有用的。
■JuluY,NagarathnaC.牙髓切除术-BMCyt测定期间暴露于牙科X线照片的儿童的遗传毒性效应的生物监测。IntJClinPediatrDent2022;15(S-1):S63-S70。
UNASSIGNED: Radiography is one of the most valuable diagnostic tools used in comprehensive dental care. Radiation from dental radiographs was thought to cause cytogenetic changes and its plausible effects can remain for some hours, months, or generations especially in children.
UNASSIGNED: To evaluate and compare the possible genotoxic effect of routinely used intraoral periapical radiographic exposure and radiovisiographic exposure in exfoliated epithelial cells as measured by the formation of micronuclei during single visit pulpectomy procedure using Buccal Micronucleus Cytome (BMCyt) assay in children.
UNASSIGNED: Study comprised 60 healthy children who has undergone either intraoral periapical radiography (IOPAR; group 1, n = 30) or radiovisiography (RVG; group 2, n = 30) during various steps of single visit pulpectomy procedure. Cytological smears were taken from the buccal mucosa immediately before the X-ray exposure and 10 ± 2 days after exposure. The cells were stained with Feulgen and evaluated for micronuclei by scoring 1,000 cells per sample.
UNASSIGNED: The genotoxic effect of radiation exposure from intraoral periapical radiography higher than that of RVG showing significant increase in micronucleus (MN) formation.
UNASSIGNED: The X-ray radiation emitted during IOPAR or RVG does induce genotoxic changes in the form of increased frequency of micronuclei. So, great care and standard protocol should be followed to advice radiographs if necessary and reduce the cumulated biological effects of radiation exposure.
UNASSIGNED: Taking into account the strong evidence of a relationship between DNA damage and carcinogenesis and the extensive application of intraoral radiographs in pediatric dentistry, it would be useful to know to what extent these dental X-rays cause genotoxic effects resulting in DNA damage on oral mucosa.
UNASSIGNED: Julu Y, Nagarathna C. Biomonitoring of Genotoxic Effect in Children Exposed to Dental Radiographs during Pulpectomy Procedure-BMCyt Assay. Int J Clin Pediatr Dent 2022;15(S-1):S63-S70.