PDF(Pubmed)
Abstract：
BACKGROUND: Cytogenotoxic damage caused by the consumption of legal and illegal drugs in drug abusers has been demonstrated, primarily due to alterations in their antioxidant capacity, cellular repair mechanisms, and increased production of free radicals. Folic acid shows antioxidant activity by acting as a reducing agent, neutralizing present free radicals, and reducing genomic damage.
METHODS: The intervention involved administering 15 mg of folic acid, divided into three doses per day, to a group of 44 drug abusers. The frequency of nuclear abnormalities (NAs) was determined; micronuclei (MNs), nuclear buds (NBUDs), binucleated cells (BNs), abnormally condensed chromatin (CC), karyorrhexis (KX), pyknotic nuclei (PNs), and karyolysis (KL) were determined at different pre-treatment (baseline) and post-treatment time points at 15 and 30 days. Additionally, a group of 44 healthy individuals was used as the control group.
RESULTS: We observed a statistically significant decrease in the frequency of NAs in the drug abuser group (28.45 ± 17.74 before supplementation vs. 11.18 ± 7.42 at 15 days and 9.11 ± 10.9 at 30 days of supplementation). Specifically, it decreased the frequency of NBUDs, BNs, CC, KX, and PNs (p < 0.05).
CONCLUSIONS: Our study demonstrates a clear improvement in cytogenotoxic damage in drug abusers supplemented with folic acid.
METHODS: The intervention involved administering 15 mg of folic acid, divided into three doses per day, to a group of 44 drug abusers. The frequency of nuclear abnormalities (NAs) was determined; micronuclei (MNs), nuclear buds (NBUDs), binucleated cells (BNs), abnormally condensed chromatin (CC), karyorrhexis (KX), pyknotic nuclei (PNs), and karyolysis (KL) were determined at different pre-treatment (baseline) and post-treatment time points at 15 and 30 days. Additionally, a group of 44 healthy individuals was used as the control group.
RESULTS: We observed a statistically significant decrease in the frequency of NAs in the drug abuser group (28.45 ± 17.74 before supplementation vs. 11.18 ± 7.42 at 15 days and 9.11 ± 10.9 at 30 days of supplementation). Specifically, it decreased the frequency of NBUDs, BNs, CC, KX, and PNs (p < 0.05).
CONCLUSIONS: Our study demonstrates a clear improvement in cytogenotoxic damage in drug abusers supplemented with folic acid.
摘要：
背景：已证明吸毒者因消耗合法和非法药物而引起的细胞遗传毒性损害，主要是由于抗氧化能力的改变，细胞修复机制，并增加了自由基的产生。叶酸通过充当还原剂而表现出抗氧化活性，中和目前的自由基，减少基因组损伤。
方法：干预措施包括给予15毫克叶酸，每天分为三剂，一群44名吸毒者。确定了核异常（NAs）的频率；微核（MNs），核芽（NBUDs），双核细胞（BNs），异常浓缩染色质（CC），karyorrhexis（KX），固缩核(PNs)，在15天和30天的不同治疗前（基线）和治疗后时间点测定核溶解（KL）。此外,44例健康者作为对照组.
结果：我们观察到药物滥用者组中NAs的频率有统计学上的显着下降（补充前28.45±17.74与在补充15天时为11.18±7.42，在补充30天时为9.11±10.9）。具体来说,它降低了NBUD的频率，BNs,CC,KX,和PNs(p<0.05)。
结论：我们的研究表明，补充叶酸的药物滥用者的细胞遗传毒性损伤有明显改善。
方法：干预措施包括给予15毫克叶酸，每天分为三剂，一群44名吸毒者。确定了核异常（NAs）的频率；微核（MNs），核芽（NBUDs），双核细胞（BNs），异常浓缩染色质（CC），karyorrhexis（KX），固缩核(PNs)，在15天和30天的不同治疗前（基线）和治疗后时间点测定核溶解（KL）。此外,44例健康者作为对照组.
结果：我们观察到药物滥用者组中NAs的频率有统计学上的显着下降（补充前28.45±17.74与在补充15天时为11.18±7.42，在补充30天时为9.11±10.9）。具体来说,它降低了NBUD的频率，BNs,CC,KX,和PNs(p<0.05)。
结论：我们的研究表明，补充叶酸的药物滥用者的细胞遗传毒性损伤有明显改善。
