METHODS: The intervention involved administering 15 mg of folic acid, divided into three doses per day, to a group of 44 drug abusers. The frequency of nuclear abnormalities (NAs) was determined; micronuclei (MNs), nuclear buds (NBUDs), binucleated cells (BNs), abnormally condensed chromatin (CC), karyorrhexis (KX), pyknotic nuclei (PNs), and karyolysis (KL) were determined at different pre-treatment (baseline) and post-treatment time points at 15 and 30 days. Additionally, a group of 44 healthy individuals was used as the control group.
RESULTS: We observed a statistically significant decrease in the frequency of NAs in the drug abuser group (28.45 ± 17.74 before supplementation vs. 11.18 ± 7.42 at 15 days and 9.11 ± 10.9 at 30 days of supplementation). Specifically, it decreased the frequency of NBUDs, BNs, CC, KX, and PNs (p < 0.05).
CONCLUSIONS: Our study demonstrates a clear improvement in cytogenotoxic damage in drug abusers supplemented with folic acid.
方法:干预措施包括给予15毫克叶酸,每天分为三剂,一群44名吸毒者。确定了核异常(NAs)的频率;微核(MNs),核芽(NBUDs),双核细胞(BNs),异常浓缩染色质(CC),karyorrhexis(KX),固缩核(PNs),在15天和30天的不同治疗前(基线)和治疗后时间点测定核溶解(KL)。此外,44例健康者作为对照组.
结果:我们观察到药物滥用者组中NAs的频率有统计学上的显着下降(补充前28.45±17.74与在补充15天时为11.18±7.42,在补充30天时为9.11±10.9)。具体来说,它降低了NBUD的频率,BNs,CC,KX,和PNs(p<0.05)。
结论:我们的研究表明,补充叶酸的药物滥用者的细胞遗传毒性损伤有明显改善。