抗生素耐药性(AMR)的迅速上升是全球关注的问题,由家禽养殖业过度使用或滥用抗生素引发。我们评估了乳酸乳球菌亚种。乳酸BIONCL17752菌株,并以其益生菌潜力为特征,可以忍受恶劣的胃肠道条件。基因组测序分析揭示了益生菌的性状,和涉及细菌素的基因簇,乳球菌A,和肌动肽生产。缺乏抗生素抗性基因,毒力,和生物胺生产表明益生菌菌株的潜力。探索了BIONCL17752菌株作为肉鸡生长促进剂的无抗生素饲料补充剂。添加4×109CFU/kg益生菌菌株的饲料,结合各种浓度的低聚果糖(FOS)1.0、2.5和5.0kg/吨,种植者,和整理者饮食,分别。体重明显改善152至171克/鸟(p<0.05),在不使用合成抗生素促进生长的情况下,实现了1.62的低饲料转化率(FCR)。生化的结果,血液学,组织学检查显示正常特征,这表明治疗对鸟的健康没有有害影响。胆固醇水平降低,甘油三酯,高密度脂蛋白(HDL),和血清中的低密度脂蛋白(LDL)是治疗鸟类健康益处的指标。家禽粪便样本的微生物群落分析显示出较高的拟杆菌丰度,Firmicutes,变形杆菌,放线菌,和镰状细菌。益生菌治疗导致肉鸡肠道中Firmicutes减少和拟杆菌(F/B比)增加,这突出了益生菌膳食补充剂的益处。重要的是,益生菌组表现出丰富的碳水化合物活性酶(CAZyme),如糖苷水解酶(GH),糖苷转移酶(GT),和碳水化合物结合模块(CBM)水解酶,它们对于复杂糖分子的降解至关重要。BIONCL17752菌株的益生菌潜力通过积极影响肠道微生物群来促进肉鸡的健康,实现最佳增长,降低死亡率,展示了益生菌在有机家禽养殖中的经济效益。
The rapid rise of antimicrobial resistance (AMR) is a global concern, being triggered by the overuse or misuse of antibiotics in poultry farming sector. We evaluated Lactococcus lactis subsp. lactis BIONCL17752 strain, and characterized its probiotic potential to endure hostile gastrointestinal conditions. Genome sequencing analysis revealed probiotics traits, and gene clusters involved in bacteriocins, lactococcin A, and sactipeptides production. The absence of genes for antibiotic resistance, virulence, and biogenic amine production indicates the potential of probiotic strain. The BIONCL17752 strain was explored for antibiotic-free feed supplement for growth promotor in broiler chicken. The feed supplemented with 4 × 109 CFU/kg of probiotic strain, in combination with various concentrations of fructooligosaccharides (FOS) 1.0, 2.5, and 5.0 kg/tonne in starter, grower, and finisher diets, respectively. A significant improvement of body weight 152 to 171 g/bird (p < 0.05), and a low feed conversion ratio (FCR) of 1.62, was achieved without using synthetic antibiotics for growth promotion. The results of biochemical, hematological, and histological examinations showed normal features, indicating that the treatment had no harmful effects on the bird\'s health. Reduced levels of cholesterol, triglycerides, high-density lipoprotein (HDL), and low-density lipoprotein (LDL) in serum are an indication of the health benefits for the treated birds. Microbial community analysis of fecal samples of poultry birds exhibited a higher abundance of Bacteroidetes, Firmicutes, Proteobacteria, Actinobacteria, and Fusobacteria. Probiotic treatment resulted in reduced Firmicutes and increased Bacteroidetes (F/B ratio) in the broiler\'s gut which highlights the benefits of probiotic dietary supplements. Importantly, the probiotic-fed group exhibited a high abundance of carbohydrate-active enzymes (CAZyme) such as glycoside hydrolases (GH), glycoside transferases (GT), and carbohydrate-binding module (CBM) hydrolases which are essential for the degradation of complex sugar molecules. The probiotic potential of the BIONCL17752 strain contributes to broilers\' health by positively affecting intestinal microbiota, achieving optimal growth, and lowering mortality, demonstrating the economic benefits of probiotic treatment in organic poultry farming.