The evaluation of the effects of architecture and design in psychiatric hospitals primarily focuses on final outcomes, such as disease progression, and is made from the perspective of evidence-based medicine. Meanwhile, the evidence-informed, realist approach addresses how the intervention works. Understanding the underlying action mechanisms of the intervention is needed to facilitate its scaling-up and adaptation in new environments. This umbrella review reports in which ways architecture and design have an effect on patients\' and staff experience in inpatient psychiatric hospital. The search was constructed around three key concepts (psychiatric hospital, design, and staff and patient outcomes) and was conducted across three reference databases (Embase, Medline, and PsychINFO). Academic and gray literature was analyzed. Information on design and architectural features in psychiatric hospitals, their effects on patients and staff experience, and the acting mechanisms enabling these effects were extracted. From 951 original references, 14 full texts were included in the analysis. Design and architectural features (e.g., aesthetic appeal of places, home-like environment) in psychiatric hospitals address patients\' stress, boost social interaction, foster patients\' autonomy and feelings of control, ensure respect for patient\'s privacy and dignity, and prevent under-and overstimulation. Using theory-driven evaluation may facilitate future hospital renovation and the evaluation of its effect.

Current biofabrication strategies are limited in their ability to replicate native shape-to-function relationships, that are dependent on adequate biomimicry of shape, size and spatial heterogeneity, within cell-laden hydrogels. In this study, a diffusion-based microfluidics platform is presented that meets these needs in a two-step process. In the first step, a hydrogel-precursor solution is dispersed into a continuous oil phase within the microfluidics tubing. By adjusting the dispersed and oil phase flow rates, the physical architecture of hydrogel-precursor phases can be adjusted to generate spherical and plug-like structures, as well as continuous meter-long hydrogel-precursor phases (up to 1.75 m). The second step involves the controlled introduction a small molecule-containing aqueous phase through a T-shaped tube connector to enable controlled small molecule diffusion across the interface of the aqueous phase and hydrogel-precursor. Application of this system is demonstrated by diffusing co-initiator sodium persulfate (SPS) into hydrogel-precursor solutions, where the controlled SPS diffusion into the hydrogel-precursor and subsequent photo-polymerization allows for the formation of unique radial stiffness patterns across the shape- and size-controlled hydrogels, as well as allowing the formation of hollow hydrogels with controllable internal architectures. Mesenchymal stromal cells are successfully encapsulated within hollow hydrogels and hydrogels containing radial stiffness gradient. The cells are observed to respond to the microscale spatial heterogeneity as evidenced by increased cell elongation in softer core regions of the hydrogel as compared to the peripheral stiffer hydrogel regions, as well as stiffness-dependent nuclear accumulation of the yes-associated protein mechano-regulator. Finally, breast cancer cells are found to phenotypically switch in response to stiffness gradients, causing a shift in their ability to aggregate, which may have implications for metastasis. The diffusion-based microfluidics will mimic native shape-to-function relationship and provides a platform to further study the roles of micro- and macroscale architectural features that exist within native tissues.

This article recounts 6 years of empirical research in a humanitarian context on spatial behaviour using the behaviour settings theory. This research journey details the shortcomings of conventional architectural processes and the subsequent development of a human-centred behaviour setting methodology that drives behaviour change for adaptable spaces. The research work puts Barker\'s theory of behaviour settings into practice to show its significant methodological abilities in shaping behaviours through spaces. While the original theory was solely an analytical account of existing behaviours in certain settings, this study marks the first pragmatic exploration of the theory into both residential and refugee contexts. The methodology that is subsequently proposed is a complementary tool to account for the deficiencies of conventional architectural design processes. A method that enables one to fully immerse themselves in the environment, recognize specific architectural interventions, assess their effects and reiterate. It is a proposal for humanizing architecture, sympathizing its processes and personalizing its results for the users of any space. This article is part of the theme issue \'People, places, things, and communities: expanding behaviour settings theory in the twenty-first century\'.

BACKGROUND: China is the hotspot of global freshwater crab diversity, but their wild populations are facing severe pressures associated with anthropogenic factors, necessitating the need to map their taxonomic and genetic diversity and design conservation policies.
RESULTS: Herein, we sequenced the mitochondrial genome of a Chinese freshwater crab species Bottapotamon fukienense, and found that it is fragmented into two chromosomes. We confirmed that fragmentation was not limited to a single specimen or population. Chromosome 1 comprised 15,111 base pairs (bp) and there were 26 genes and one pseudogene (pseudo-nad1) encoded on it. Chromosome 2 comprised 8,173 bp and there were 12 genes and two pseudogenes (pseudo-trnL2 and pseudo-rrnL) encoded on it. Combined, they comprise the largest mitogenome (23,284 bp) among the Potamidae. Bottapotamon was the only genus in the Potamidae dataset exhibiting rearrangements of protein-coding genes. Bottapotamon fukienense exhibited average rates of sequence evolution in the dataset and did not differ in selection pressures from the remaining Potamidae.
CONCLUSIONS: This is the first experimentally confirmed fragmentation of a mitogenome in crustaceans. While the mitogenome of B. fukienense exhibited multiple signs of elevated mitogenomic architecture evolution rates, including the exceptionally large size, duplicated genes, pseudogenisation, rearrangements of protein-coding genes, and fragmentation, there is no evidence that this is matched by elevated sequence evolutionary rates or changes in selection pressures.

Amino acids are necessary nutrients for the growth of Oryza sativa (rice), which can be mediated by amino acid transporter; however, our understanding of these transporters is still limited. This study found that the expression levels of amino acid permease gene OsAAP12 differed between indica and japonica rice. Altered expression of OsAAP12 negatively regulated tillering and yield in transgenic rice lines. Subcellular localization revealed that OsAAP12 was primarily localized to the plasma membrane. Moreover, it was indicated that OsAAP12 transported polar neutral amino acids asparagine (Asn), threonine (Thr), and serine (Ser) through experiments involving yeast heterologous complementation, fluorescence amino acid uptake, and amino acid content determination. Additionally, exogenous application of amino acids Asn, Thr, and Ser suppressed axillary buds outgrowth in OsAAP12 overexpression lines compared with wild-type ZH11. Conversely, the opposite trend was observed in CRISPR mutant lines. RNA-seq analysis showed that the expression patterns of genes involved in the nitrogen and cytokinin pathways were generally altered in OsAAP12 modified lines. Hormone assays indicated that OsAAP12 mutant lines accumulated cytokinins in the basal part of rice, whereas overexpression lines had the opposite effect. In summary, CRISPR mutant of OsAAP12 boosted rice tillering and grain yield by coordinating the content of amino acids and cytokinins, which has potential application value in high-yield rice breeding.

The home and neighborhood environments impact the social and mental health of older adults, yet little research has addressed the various contexts that can affect these relationships, such as community culture, built and natural elements, and demographics. This survey-based study examined community-dwelling older adults\' access and use of transitional outdoor/indoor space (i.e., porches, gardens, windows, etc.), and how that use was related to health variables and changed with the pandemic in two available samples of older adults in the United States and Italy. Use of both outdoor and indoor space was found to be more individualistic in Boston, in the United States, than in Chieti, Italy, where use of these areas with others was more common. Results suggest that window viewing from within the home may be an activity that individuals in Italy engage in when feeling lonely. Changes in the use of home and community space after COVID-19 were minimal; only in the United States did individuals report greater time indoors since the onset of the pandemic. Use of the built environment in and around the home by older adults was found to have multidimensional characteristics between the United States and Italy, with the potential to foster connections and improve well-being.

The motivations for incorporating nature into the design of cities have never been more compelling. Creating experiences with nature that occur every day (everyday nature) in cities could help reverse the fate of many threatened species and connect people with nature and living cultural traditions. However, this requires more than just urban greening; it involves ensuring daily doses of nature in a way that also supports nonhuman organisms. A major shift in the way nature is conceived of and is made part of the design of cities is required. Principles include reconsidering nature as a development opportunity rather than a constraint and eliminating offsetting of biodiversity site values. Processes include using biodiversity-sensitive design frameworks and establishing meaningful professional engagement among ecologists, planners, and designers. Challenges include design obstacles, conflicts between nature and people (e.g., safety, disease, and noise) that require careful management, and socioeconomic and political considerations (e.g., Global North vs. Global South). Research to interrogate the multiple benefits of nature in cities can complement experimental interventions, ultimately supporting better urban design and creating much more resiliently built environments for people and nature.
Diseño de ciudades para la naturaleza cotidiana Resumen Los motivos para incorporar a la naturaleza dentro del diseño urbano jamás habían sido tan convincentes. La creación en las ciudades de experiencias con la naturaleza que ocurren a diario (naturaleza cotidiana) podría ayudar a cambiar el destino de muchas especies amenazadas y conectar a las personas con la naturaleza y las tradiciones culturales vivientes. Lo anterior requiere más que reverdecimiento urbano ya que involucra dosis diarias de naturaleza de manera que también mantengan a los organismos no humanos. Se necesita de un cambio mayor en la manera en la que se concibe a la naturaleza y cómo se le hace parte del diseño urbano. Los principios incluyen reconsiderar a la naturaleza como una oportunidad de desarrollo en lugar de una limitación y eliminar la compensación del valor de los sitios de biodiversidad. Los procesos incluyen el uso de marcos de diseños sensibles con la biodiversidad y el establecimiento de una participación profesional significativa entre los ecologistas, los planeadores y los diseñadores. Los retos incluyen los obstáculos del diseño, conflictos entre la naturaleza y las personas (seguridad, enfermedades y ruido) que requieren de un manejo cuidadoso y consideraciones políticas (Norte Global versus Sur Global). La investigación para interrogar los múltiples beneficios de la naturaleza en las ciudades puede complementar a las intervenciones, a la larga respaldando un mejor diseño urbano y creando ambientes para las personas y la naturaleza construidos con mayor resiliencia.

OBJECTIVE: A novel architecture-based grading system for pancreatic ductal adenocarcinoma (PDAC) is tested against traditional grading.
METHODS: A total of 103 PDAC resections were graded by College of American Pathologists/American Joint Committee on Cancer (CAP/AJCC) guidelines and by a system using an architectural pattern (dispersed larger duct = low grade vs dense smaller duct = high grade). Survival analyses and interobserver variability were assessed. In total, 114 cases from a public data set were used for validation.
RESULTS: Median overall survivals were 15 and 36 months for architectural high-grade and low-grade cases, respectively (P < .001). Conversely, CAP/AJCC grading showed no survival difference between well-differentiated and moderately differentiated tumors (P = .545). Architecture-based grading remained prognostically significant for recurrence-free survival (P = .004), but CAP/AJCC grading was not (P = .226). Adjusted for stage and margin status, architectural high-grade PDACs showed a hazard ratio of 2.69 relative to low grade (P < .001) for survival. The validation cohort confirmed prognostic differences in overall (P < .001) and recurrence-free survival (P = .027) for the architecture-based system, outperforming CAP/AJCC grading. Architecture-based grading exhibited a Cohen\'s ĸ value of 0.710 (substantial agreement), superior to traditional grading (0.488, moderate agreement).
CONCLUSIONS: Grading PDAC based on architectural pattern results in superior prognostication and reproducibility vs CAP/AJCC grading.

Water transport, mechanical support and storage are the vital functions provided by the xylem. These functions are carried out by different cells, exhibiting significant anatomical variation not only within species but also within individual trees. In this study, we used a comprehensive dataset to investigate the consistency of predicted hydraulic vessel diameter widening values in relation to the distance from the tree apex, represented by the relationship Dh ∝ Lβ (where Dh is the hydraulic vessel diameter, L the distance from the stem apex and β the scaling exponent). Our analysis involved 10 Fagus sylvatica L. trees sampled at two distinct sites in the Italian Apennines. Our results strongly emphasize that vessel diameter follows a predictable pattern with the distance from the stem apex and β ~ 0.20 remains consistent across cambial age and climates. This finding supports the hypothesis that trees do not alter their axial configuration represented by scaling of vessel diameter to compensate for hydraulic limitations imposed by tree height during growth. The study further indicates that within-tree variability significantly contributes to the overall variance of the vessel diameter-stem length exponent. Understanding the factors that contribute to the intraindividual variability in the widening exponent is essential, particularly in relation to interspecific responses and adaptations to drought stress.

BACKGROUND: The aim of this article is to attempt to answer the question of how to enhance the fulfillment of needs for senior citizens residing in riverside cities. To achieve this, an attempt was made to develop the principles of a cohesive system that enables the activation of waterfront areas located in urbanized regions, often affected by a deficit of green spaces. The concept presented in this article is based on a consistent focus on architectural and urban design solutions that provide opportunities for functional enrichment of underutilized riverside areas for recreational purposes.
METHODS: Based on literature studies, field research, and design analysis, this article demonstrates the possibility of taking a structural approach to implementing changes in the utilization of green spaces located by the water in contemporary cities. Using the results of the analysis, an original system called the mobile architectural-urban elements (mobilne elementy architektoniczno-urbanistyczne - MEAU) was developed to activate the untapped potential of waterfront areas to meet the specific needs of senior citizens.
RESULTS: The research objective outlined in the introduction led to the development of a solution based on the utilization of floating architecture for the establishment of services and amenities that enable comfortable and diverse leisure activities for the elderly. Additionally, it was demonstrated that the described approach aligns with the multidimensional vision of improving the well-being of users, as defined by the European Commission in the New European Bauhaus (NEB) program.
CONCLUSIONS: The analysis conducted in this article allows for preliminary confirmation of the hypothesis that the specific needs of senior citizens can be fulfilled through the activation of waterfront areas using MEAU located on the water. Such actions not only activate existing resources but also align with the guidelines of the NEB idea, providing a coherent and applicable model with significant implementation potential for most waterfront cities. Med Pr Work Health Saf. 2024;75(3):211-222.
UNASSIGNED: Celem artykułu jest próba odpowiedzi na pytanie, w jaki sposób można podwyższyć poziom spełnienia potrzeb osób w wieku senioralnym zamieszkujących miasta nadrzeczne. W tym celu podjęto próbę opracowania zasad funkcjonowania spójnego systemu umożliwiającego aktywizację terenów nadwodnych zlokalizowanych w obszarach zurbanizowanych dotkniętych często deficytem obszarów zielonych. Koncepcja przedstawionego w artykule systemu bazuje na konsekwentnym ukierunkowaniu rozwiązań architektoniczno-urbanistycznych na zapewnienie możliwości wzbogacenia funkcjonalnego niewykorzystywanych w pełni dla potrzeb rekreacji i wypoczynku terenów nadrzecznych.
UNASSIGNED: Bazując na studiach literatury przedmiotu, badaniach terenowych i analizach projektowych, wykazano możliwość strukturalnego podejścia do wdrożenia zmian w sposobie użytkowania terenów zielonych zlokalizowanych nad wodą we współczesnym mieście. Na podstawie wyników analiz opracowano autorski system użycia tzw. mobilnych elementów architektoniczno-urbanistycznych (MEAU), zlokalizowanych na wodzie, do aktywizacji niewykorzystanego w pełni potencjału terenów nadwodnych w celu realizacji szczególnych potrzeb seniorów.
UNASSIGNED: Postawiony na wstępie cel badawczy doprowadził do opracowania rozwiązania opartego na wykorzystaniu idei tzw. architektury pływającej dla potrzeb lokalizacji usług i udogodnień pozwalających na komfortowe i zróżnicowane spędzanie czasu wolnego przez osoby w wieku podeszłym. Na przykładzie Wrocławia wskazano potencjalną mapę lokalizacji elementów systemu, które bazując na efekcie synergii, mogą w znaczący sposób zmienić postrzeganie istotnych fragmentów miasta w oczach zarówno jego mieszkańców, jak i gości chcących odwiedzić je w celach turystycznych. Wykazano również, że opisywane działanie wpisuje się w wielowymiarową wizję poprawy dobrostanu użytkowników zdefiniowaną przez Komisję Europejską w programie tzw. Nowego Europejskiego Bauhausu (NEB).
UNASSIGNED: Przeprowadzona w artykule analiza pozwala na wstępne potwierdzenie przyjętej tezy, zgodnie z którą możliwe jest spełnienie szczególnych potrzeb osób w wieku senioralnym poprzez aktywizację terenów nadrzecznych przy wykorzystaniu do tego celu MEAU zlokalizowanych na wodzie. Działanie takie poprzez aktywizację posiadanych zasobów wpisuje się także wielowymiarowo w wytyczne idei NEB, stanowiąc spójny, możliwy do zastosowania w większości miast położonych nad wodą, wzór postępowania o dużym potencjale wdrożeniowym. Med Pr Work Health Saf. 2024;75(3):211–222.