抗生素抗性微生物的发展促使研究可能的抗生素替代品。因此,本研究的目的是评估饮食螺旋藻提取物作为抗生素替代品对日本鹌鹑(Coturnixjaponica)生长的影响,抗氧化状态,血液参数,和盲肠微生物。这项研究总共使用了150只日本鹌鹑,平均分为5个实验组(每组10只鸟,重复3个):第1组(G1)接受了不含任何S.platensis提取物的基础饮食,第2组(G2)接受补充有1mL板条提取物/kg的基础饮食,第3组(G3)接受补充有2mL板条提取物/kg的基础饮食,第4组(G4)接受补充有3mL板条提取物/kg的基础饮食,和第5组(G5)从第7天开始至第35天接受补充4mL板条提取物/kg的基础饮食。结果表明,与G1期的对照鸟类相比,添加4mLS.platensis提取物/kg日粮(G5)的日本鹌鹑的活体重明显更好,体重增加,饲料摄入量,饲料转化率,消化酶,血液参数,肝肾功能,血脂谱,抗氧化剂概况,免疫学参数,和盲肠微生物计数。尸体的百分比没有显着变化,肝脏,和所有5组的内脏总量。与G1中的鸟类相比,G2中只有g百分比显着增加。此外,与G1中的鸟类相比,肠道pH值在G2和G5中显示出显着下降。煮完鹌鹑肉之后,多汁性和嫩度随着鸭嘴草提取物水平的增加而增加,而随着S.platensis提取物含量的增加,香气和味道略有下降。此外,当使用高浓度的S.platensis提取物时,肉的亮度降低,而红色和黄色增加。纸片扩散实验表明,板条提取物对金黄色葡萄球菌具有显著的抗菌活性,单核细胞增生李斯特菌,空肠弯曲杆菌,伤寒沙门氏菌,抑制区范围从16到42毫米。这种活性可能归因于S.platensis提取物中的挥发性化学物质,其中土豆素和2-甲基异冰片是主要成分。在日本鹌鹑的饮食中,可以得出结论,S.platensis的提取物可以用作饲料添加剂和抗生素的替代品。
The development of antibiotic-resistant microorganisms prompted the investigation of possible antibiotic substitutes. As a result, the purpose of the current study is to assess the effect of dietary Spirulina platensis extract as an antibiotic alternative on Japanese quail (Coturnix japonica) growth, antioxidant status, blood parameters, and cecal microorganisms. There was a total of 150 Japanese quails used in this study, divided equally among 5 experimental groups (10 birds per group with 3 replicates): group 1 (G1) received a basal diet without any S. platensis extract, group 2 (G2) received a basal diet supplemented with 1 mL S. platensis extract/kg, group 3 (G3) received a basal diet supplemented with 2 mL S. platensis extract/kg, group 4 (G4) received a basal diet supplemented with 3 mL S. platensis extract/kg, and group 5 (G5) received a basal diet supplemented with 4 mL S. platensis extract/kg from d 7 until d 35. The results showed that compared to the control birds in G1, Japanese quail supplemented with 4 mL of S. platensis extract/kg of diet (G5) had significantly better live body weight, body weight gain, feed intake, feed conversion ratio, digestive enzymes, blood parameters, liver and kidney functions, lipid profile, antioxidant profile, immunological parameters, and cecal microorganism\'s count. There were no significant changes in the percentage of carcasses, liver, and total giblets among all the 5 groups. Only gizzard percentage showed a significant increase in G2 compared to birds in G1. In addition, intestinal pH showed a significant drop in G2 and G5 compared to birds in G1. After cooking the quail meat, the juiciness and tenderness increased as S. platensis extract levels increased, whereas aroma and taste declined slightly as S. platensis extract levels increased. Furthermore, when a high concentration of S. platensis extract was used, the lightness of the meat reduced while its redness and yellowness increased. The disk diffusion assay showed that S. platensis extract had significant antibacterial activity against Staphylococcus aureus, Listeria monocytogenes, Campylobacter jejuni, and Salmonella typhi, with inhibition zones ranging from 16 to 42 mm. This activity may be attributable to the volatile chemicals in S. platensis extract, of which Geosmin and 2-methylisoborneol are the primary components. In the diet of Japanese quails, it is possible to draw the conclusion that the extract of S. platensis can be utilized as a feed additive and as an alternative to antibiotics.