PDF(Pubmed)
Abstract：
Edwardsiella piscicida causes significant economic losses to the aquaculture industry worldwide. Phage-based biocontrol methods are experiencing a renaissance because of the spread of drug-resistant genes and bacteria resulting from the heavy use of antibiotics. Here, we showed that the novel Edwardsiella phage EPP-1 could achieve comparable efficacy to florfenicol using a zebrafish model of Edwardsiella piscicida infection and could reduce the content of the floR resistance gene in zebrafish excreta. Specifically, phage EPP-1 inhibited bacterial growth in vitro and significantly improved the zebrafish survival rate in vivo (P = 0.0035), achieving an efficacy comparable to that of florfenicol (P = 0.2304). Notably, integrating the results of 16S rRNA sequencing, metagenomic sequencing, and qPCR, although the effects of phage EPP-1 converged with those of florfenicol in terms of the community composition and potential function of the zebrafish gut microbiota, it reduced the floR gene content in zebrafish excreta and aquaculture water. Overall, our study highlights the feasibility and safety of phage therapy for edwardsiellosis control, which has profound implications for the development of antibiotic alternatives to address the antibiotic crisis.
摘要：
比西西氏菌给全球水产养殖业造成了巨大的经济损失。基于噬菌体的生物防治方法正在经历复兴，因为大量使用抗生素导致耐药基因和细菌的传播。这里,我们表明，使用斑马鱼感染Edwardsiella感染的斑马鱼模型，新型Edwardsiella噬菌体EPP-1可以达到与氟苯尼考相当的功效，并且可以降低斑马鱼排泄物中floR抗性基因的含量。具体来说,噬菌体EPP-1在体外抑制细菌生长,显著提高斑马鱼体内存活率(P=0.0035),达到与氟苯尼考相当的疗效（P=0.2304）。值得注意的是,整合16SrRNA测序的结果，宏基因组测序，和qPCR，尽管在斑马鱼肠道菌群的群落组成和潜在功能方面，噬菌体EPP-1的作用与氟苯尼考的作用趋同，它降低了斑马鱼排泄物和养殖水体中的floR基因含量。总的来说,我们的研究强调了噬菌体疗法控制爱德华病的可行性和安全性，这对开发抗生素替代品以解决抗生素危机具有深远的影响。
