使用唾液作为来自猪的生物样品具有很高的实际意义,因为从猪的血液收集是困难的和有压力的。在这项研究中,两种不同材料的影响,棉卷和聚丙烯海绵,在猪唾液采集中进行了评估。为此,在一组13种分析物中评估了用于采样的材料的效果,包括与压力有关的那些(皮质醇和催产素),炎症和免疫(腺苷脱氨酶,触珠蛋白和髓过氧化物酶),氧化还原稳态(唾液还原铜的能力,唾液的铁还原活性,和Trolox等效的抗氧化能力),和败血症(降钙素原),以及与代谢和不同组织和器官相关的其他常规分析物,如乳酸脱氢酶,肌酸激酶,尿素,和总蛋白质浓度。聚丙烯海绵提供比棉卷更高的样品体积。尽管两种材料的一些唾液分析物的结果相同,其他分析物,如肌酸激酶,触珠蛋白和总蛋白,显示显着差异,这取决于用于唾液收集的材料。因此,在解释唾液分析物的分析结果时,应考虑猪唾液收集材料的类型。
The use of saliva as a biological sample from pigs is of high practical interest because blood collection from pigs is difficult and stressful. In this study, the influence of two different materials, a cotton roll and a polypropylene sponge, in porcine saliva collection was evaluated. For this purpose, the effect of the material used for sampling was evaluated in a panel of 13
analytes, including those related to stress (cortisol and oxytocin), inflammation and immunity (adenosine deaminase, haptoglobin and myeloperoxidase), redox homeostasis (the cupric reducing ability of saliva, the ferric reducing activity of saliva, and the Trolox equivalent antioxidant capacity), and sepsis (procalcitonin), as well as other routine
analytes related to metabolism and different tissues and organs, such as lactate dehydrogenase, creatine kinase, urea, and total protein concentration. The polypropylene sponge provided a higher sample volume than the cotton roll. Although the results of some salivary
analytes were equivalent for both materials, other
analytes, such as creatine kinase, haptoglobin and total proteins, showed significant differences depending on the material used for saliva collection. Therefore, the type of material used for salivary collection in pigs should be considered when interpreting the results of analyses of the salivary
analytes.