背景:涎腺腺样囊性癌(ACC)的特征是高侵袭性和缓慢生长的模式,其病因仍不明。雷公藤内酯(TN)已证明作为抗ACC的药物治疗剂的功效。尽管如此,TN治疗ACC的具体靶点和分子作用机制尚未阐明.
目的:通过将网络药理学与实验室实验相结合,这项研究探讨了与TN治疗ACC相关的前瞻性靶标和分子机制。
方法:最初,从公共数据库中获得了与TN相对于ACC的相关目标。随后,网络药理学和生物信息学分析的结合被用于筛选TN治疗ACC的前10个中心靶标和关键信号通路.最后,进行了涉及各种分子测定的体外实验,以评估TN处理后细胞的生物学表型,包括对细胞凋亡水平的评估,平板迁移,和其他参数,从而验证关键基因和途径。
结果:确定了总共23个与ACC相关的TN目标,前10个hub基因是MAPK8,PTGS2,RELA,MAPK14,NR3C1,HDAC1,PPARG,NFKBIA,AR,和PGR。TNF信号通路与TN治疗ACC之间存在显著的相关性。体外实验表明,TN处理可提高RELA磷酸化,同时降低MAPK14磷酸化并诱导G2/M阻滞。TN表现出通过增加caspase-3活性来提高细胞凋亡率的能力,活性氧(ROS)水平升高,线粒体功能障碍,和抑制细胞迁移。
结论:TN通过激活TNF信号通路在ACC治疗中具有潜在的治疗作用。在确定的候选人中,MAPK8,HDAC1,PTGS2,RELA,NR3C1,PPARG,NFKBIA,AR,在ACC治疗的背景下,PGR和PGR成为TN最相关的治疗靶标。
BACKGROUND: Salivary Adenoid Cystic Carcinoma (ACC) is characterized by a highly invasive and slow-growing pattern, and its etiology remains unidentified. Triptonide (TN) has demonstrated efficacy as a pharmacotherapeutic agent against ACC. Nonetheless, the specific targets and mechanism of molecular action underlying the effectiveness of TN in treating ACC have not been elucidated.
OBJECTIVE: By integrating network pharmacology with in-laboratory experiments, this research delves into the prospective targets and molecular mechanisms associated with the application of TN in treating ACC.
METHODS: Initially, pertinent targets associated with TN against ACC were acquired from public databases. Subsequently, a combination of network pharmacology and bioinformatics analysis was utilized to screen the top 10 hub targets and key signal pathways of TN-treating ACC. Finally, in vitro experiments involving various molecular assays were conducted to evaluate the biological phenotypes of cells following TN treatment, encompassing assessments of apoptosis levels, plate migration, and other parameters, thereby validating pivotal genes and pathways.
RESULTS: A total of 23 pertinent targets for TN in relation to ACC were identified, with the top 10 hub genes being MAPK8, PTGS2, RELA, MAPK14, NR3C1, HDAC1, PPARG, NFKBIA, AR, and PGR. There was a significant correlation between the TNF signaling pathway and the treatment of ACC with TN. In vitro experiments demonstrated that TN treatment elevated RELA phosphorylation while concurrently reducing MAPK14 phosphorylation and inducing G2/M arrest. TN exhibited the ability to enhance the apoptosis rate through increased caspase-3 activity, elevated levels of Reactive Oxygen Species (ROS), mitochondrial dysfunction, and inhibition of cell migration.
CONCLUSIONS: There is a potential therapeutic role for TN in the treatment of ACC through the activation of the TNF signaling pathway. Among the identified candidates, MAPK8, HDAC1, PTGS2, RELA, NR3C1, PPARG, NFKBIA, AR, and PGR emerge as the most pertinent therapeutic targets for TN in the context of ACC treatment.