Abstract：
OBJECTIVE: To evaluate and compare the expression of E-cadherin, Snail1 and Twist1 in pleomorphic adenomas (PAs), adenoid cystic carcinomas (AdCCa) and carcinoma ex-pleomorphic adenomas (CaexPA) of salivary glands, as well as investigate possible associations with clinicopathological parameters.
METHODS: E-cadherin, Snail1 and Twist1 antibody immunostaining were analyzed semiquantitatively in 20 PAs, 20 AdCCas and 10 CaexPAs. Cases were classified as low and high expression for analysis of the association with clinicopathological parameters.
RESULTS: Compared to PAs, AdCCas and CaexPAs exhibited higher nuclear expression of Snail1 (p = 0.021 and p = 0.028, respectively) and Twist1 (p = 0.009 and p = 0.001). Membranous and cytoplasmic expression of E-cadherin were positively correlated in PAs, AdCCas and CaexPAs (r = 0.645, p = 0.002; r = 0.824, p < 0.001; r = 0.677, p = 0.031). In PAs, positive correlation was found between nuclear expression of Snail1 and membrane expression of E-cadherin (r = 0.634; p = 0.003), as well as between nuclear expression of Snail1 and Twist1 (r = 0.580; p = 0.007). Negative correlations were detected between membrane expression of E-cadherin and cytoplasmic expression of Snail1 in AdCCas (r = - 0.489; p = 0.029).
CONCLUSIONS: E-cadherin, Twist1, and Snail1 may participate in modulating events related to cell differentiation and adhesion in PAs and to biological behavior in AdCCas and CaexPAs, which indicates the involvement of EMT in these processes. Furthermore, the expression of these proteins in these carcinomas may reflect the plasticity feature of EMT.
METHODS: E-cadherin, Snail1 and Twist1 antibody immunostaining were analyzed semiquantitatively in 20 PAs, 20 AdCCas and 10 CaexPAs. Cases were classified as low and high expression for analysis of the association with clinicopathological parameters.
RESULTS: Compared to PAs, AdCCas and CaexPAs exhibited higher nuclear expression of Snail1 (p = 0.021 and p = 0.028, respectively) and Twist1 (p = 0.009 and p = 0.001). Membranous and cytoplasmic expression of E-cadherin were positively correlated in PAs, AdCCas and CaexPAs (r = 0.645, p = 0.002; r = 0.824, p < 0.001; r = 0.677, p = 0.031). In PAs, positive correlation was found between nuclear expression of Snail1 and membrane expression of E-cadherin (r = 0.634; p = 0.003), as well as between nuclear expression of Snail1 and Twist1 (r = 0.580; p = 0.007). Negative correlations were detected between membrane expression of E-cadherin and cytoplasmic expression of Snail1 in AdCCas (r = - 0.489; p = 0.029).
CONCLUSIONS: E-cadherin, Twist1, and Snail1 may participate in modulating events related to cell differentiation and adhesion in PAs and to biological behavior in AdCCas and CaexPAs, which indicates the involvement of EMT in these processes. Furthermore, the expression of these proteins in these carcinomas may reflect the plasticity feature of EMT.
摘要：
目的：评估和比较E-cadherin的表达，多形性腺瘤（PAs）中的Snail1和Twist1，涎腺腺样囊性癌（AdCCa）和多形性腺瘤（CaexPA），以及调查与临床病理参数的可能关联。
方法：E-cadherin，在20个PA中对Snail1和Twist1抗体免疫染色进行了半定量分析，20个AdCCas和10个CaexPA。为了分析与临床病理参数的相关性，将病例分为低表达和高表达。
结果：与PA相比，AdCCas和CaexPAs表现出更高的Snail1（分别为p=0.021和p=0.028）和Twist1（p=0.009和p=0.001）的核表达。E-cadherin在PAs中的膜和细胞质表达呈正相关，AdCCas和CaexPAs(r=0.645,p=0.002;r=0.824,p<0.001;r=0.677,p=0.031)。在PA中，Snail1的核表达与E-cadherin的膜表达呈正相关(r=0.634;p=0.003),以及Snail1和Twist1的核表达之间（r=0.580；p=0.007）。AdCCas中E-cadherin的膜表达与Snail1的细胞质表达呈负相关（r=-0.489；p=0.029）。
结论：E-cadherin，Twist1和Snail1可能参与调节与PAs中的细胞分化和粘附以及AdCCas和CaexPAs中的生物学行为有关的事件，这表明EMT参与了这些过程。此外，这些蛋白质在这些癌中的表达可能反映了EMT的可塑性特征。
方法：E-cadherin，在20个PA中对Snail1和Twist1抗体免疫染色进行了半定量分析，20个AdCCas和10个CaexPA。为了分析与临床病理参数的相关性，将病例分为低表达和高表达。
结果：与PA相比，AdCCas和CaexPAs表现出更高的Snail1（分别为p=0.021和p=0.028）和Twist1（p=0.009和p=0.001）的核表达。E-cadherin在PAs中的膜和细胞质表达呈正相关，AdCCas和CaexPAs(r=0.645,p=0.002;r=0.824,p<0.001;r=0.677,p=0.031)。在PA中，Snail1的核表达与E-cadherin的膜表达呈正相关(r=0.634;p=0.003),以及Snail1和Twist1的核表达之间（r=0.580；p=0.007）。AdCCas中E-cadherin的膜表达与Snail1的细胞质表达呈负相关（r=-0.489；p=0.029）。
结论：E-cadherin，Twist1和Snail1可能参与调节与PAs中的细胞分化和粘附以及AdCCas和CaexPAs中的生物学行为有关的事件，这表明EMT参与了这些过程。此外，这些蛋白质在这些癌中的表达可能反映了EMT的可塑性特征。
