在水产养殖中,波动的水温可以作为一个强大的压力源,影响病原菌的毒力和传播动态,可能引发疫情并影响鱼类健康。这项工作的目的是检查Shewanellaspp的影响。血液学感染,生物化学,不同水温下尼罗罗非鱼(Oreochromisniloticus)的抗氧化免疫参数。为此,将180条鱼一式三份分成6组(每组30条鱼;每次重复10条鱼)。第1组(G1),G2和G3在不同的水温(22°C,28°C,和31°C,分别)没有感染。而G4,G5和G6是用0.2mL的希瓦氏菌进行IP注射。(0.14x105)并在22°C下饲养,28°C,和31°C,分别。Shewanellaspp.感染导致血液学参数(红细胞和白细胞,血红蛋白,和堆积细胞体积%)和免疫-抗氧化反应(吞噬活性%,吞噬指数,溶菌酶,一氧化氮),总抗氧化能力,过氧化氢酶,还原型谷胱甘肽,尤其是在22°C此外,肝肾功能指标(丙氨酸转氨酶,天冬氨酸转氨酶,尿素,和肌酐),应激生物标志物(葡萄糖和皮质醇),丙二醛,和促炎细胞因子(白细胞介素-1β和肿瘤坏死因子-α)是希瓦氏菌的后果。感染,尤其是在22°CShewanellaspp。感染在肝和肾组织中表现出明显的组织病理学变化。值得的是,Shewanellaspp.会导致尼罗罗非鱼血液学的有害改变,生物化学,以及各种水温下的抗氧化免疫参数,但是在22°C的水温下观察到了主要的有害变化。因此,我们可以得出结论,希瓦氏菌的感染动力学。在22°C时被夸大。这些结果可能有助于了解尼罗罗非鱼中这种感染的性质。
In aquaculture, fluctuating water temperatures can act as a potent stressor, influencing the virulence and transmission dynamics of pathogenic bacteria, potentially triggering outbreaks and impacting fish health. The purpose of this work was to examine the impact of Shewanella spp. infection on hematological, biochemical, and antioxidant-immune parameters of Nile tilapia (Oreochromis niloticus) under different water temperatures. For this purpose, 180 fish were divided into 6 groups in triplicate (30 fish per group; 10 fish per replicate). Group 1 (G1), G2, and G3 were reared at varying water temperatures (22 °C, 28 °C, and 31 °C, respectively) without infection. While G4, G5, and G6 were IP-injected with 0.2 mL of Shewanella spp. (0.14 × 105) and reared at 22 °C, 28 °C, and 31 °C, respectively. Shewanella spp. infection induced significant lowering (p < 0.05) in hematological parameters (red and white blood cells, hemoglobin, and packed cell volume%) and immune-antioxidant responses (phagocytic activity%, phagocytic index, lysozyme, nitric oxide), total antioxidant capacity, catalase, and reduced glutathione, especially at 22 °C. Moreover, a significant increase (p < 0.05) in the hepato-renal function indicators (alanine aminotransferase, aspartate aminotransferase, urea, and creatinine), stress biomarkers (glucose and cortisol), malondialdehyde, and pro-inflammatory cytokines (interleukin-1β and tumor necrosis factor-α) were the consequences of the Shewanella spp. infection, especially at 22 °C. The Shewanella spp. infection exhibited marked histopathological changes in the hepatic and renal tissues. Worthily, Shewanella spp. can cause detrimental alterations in Nile tilapia\'s hematological, biochemical, and antioxidant-immune parameters at various water temperatures, but the major detrimental changes were observed at a water temperature of 22 °C. Consequently, we can conclude that the infection dynamics of Shewanella spp. are exaggerated at 22 °C. These outcomes could help in understanding the nature of such an infection in Nile tilapia.