■为治疗急性阑尾炎而切除的阑尾中约有15%-25%呈现正常的宏观和宏观形态。这项研究的目的是验证促炎,从具有临床实验室和影像学特征的急性阑尾炎患者中切除了形态学正常阑尾的神经内分泌和免疫介质。
■从121名两种性别的成年患者中摘除的阑尾根据其以下特征分为三组:第1组:来自临床患者的53例宏观和微观正常阑尾,急性阑尾炎的实验室和影像学诊断;第2组:来自临床,实验室,急性阑尾炎的影像学和组织病理学诊断;第3组:接受右结肠切除术治疗局限性升结肠腺癌的患者中的44例正常阑尾。所有附录都进行了胃泌素抑制剂肽的免疫组织化学研究,肥大细胞类胰蛋白酶,血管内皮生长因子;肠血管活性肽,肿瘤坏死因子α,白细胞介素1,前列腺素E2,基因蛋白产物9.5,CD8T淋巴细胞,突触素,烯醇化酶,S100蛋白
■第1组显示突触素水平升高,烯醇化酶,肥大细胞类胰蛋白酶和PGP-9.5与其他两组比较。与其他两组相比,第2组的白介素1,CD8T淋巴细胞和前列腺素E2水平升高。第3组证实了所有这些神经内分泌的正常水平,免疫和促炎介质。
■从临床和补充检查表明急性阑尾炎患者切除的形态正常阑尾有与介体突触素相关的阑尾神经免疫内分泌紊乱,烯醇化酶,肥大细胞相关的类胰蛋白酶和基因蛋白产物9.5。
UNASSIGNED: About 15%-25% of appendices removed to treat acute appendicitis present normal macro- and macroscopic morphology. The objective of this study was to verify an association of proinflammatory, neuroendocrine and immune mediators with morphologically normal appendices removed from patients with clinical laboratorial and imaging characteristics of acute appendicitis.
UNASSIGNED: Appendices removed from 121 adult patients of both genders were distributed into three groups according to their following characteristics: group 1: 53 macro- and microscopically normal appendices from patients with clinical, laboratorial and imaging diagnosis of acute appendicitis; group 2: 24 inflamed appendices from patients with clinical, laboratorial, imaging and histopathological diagnosis of acute appendicitis; group 3: 44 normal appendices from patients submitted to right colectomy to treat localized ascending colon adenocarcinoma. All appendices were immunohistochemically studied for gastrin inhibitor peptide, mast cell tryptase, vascular endothelial growth factor; intestinal vasoactive peptide, tumor necrosis factor alpha, interleukin 1, prostaglandin E2, gene-protein product 9.5, CD8 T lymphocytes, synaptophysine, enolase, and S100 protein.
UNASSIGNED: The group 1 revealed increased levels of synaptophysine, enolase, mast cell tryptase and PGP-9.5 comparing with the other two groups. The group 2 presented increased levels of interleukin 1, CD8 T lymphocytes and prostaglandin E2 comparing with the other two groups. The group 3 confirmed the normal levels of all these neuroendocrine, immune and proinflammatory mediators.
UNASSIGNED: Morphologically normal appendices removed from patients with clinical and complementary exams indicating acute appendicitis have appendicular neuroimmunoendocrine disorder associated with the mediators synaptophysin, enolase, mast cell-related tryptase and gene-protein product 9.5.