未经证实:高度保守的管状蛋白(TLP)在动物神经元发育和植物生长中起关键作用。TLPs的抗逆性功能在植物中得到了广泛的研究,然而,关于作物内部TLP的比较研究知之甚少。
未经授权:生物信息学鉴定,系统发育分析,顺式元素分析,表达分析,顺式元素分析,对多种作物的TLP基因家族进行了分析。
未经批准:在这项研究中,对7种作物的TLP基因进行了综合分析,以探讨其他作物是否可以实现水稻中TLP的相似功能。我们在甘氨酸max中鉴定了20、9、14、11、12、35、14和13个TLP基因,大麦,双色高粱,拟南芥,水稻,普通小麦,Setaria斜体和Zeamays,分别。根据氨基酸,将它们全部分为两组和十个正统(Ors)。大多数TLP基因有两个结构域,tubby-like域和F-box域,而Or5的成员只有管状域。此外,Or5有更多的外显子和更短的DNA序列,表明不同Ors的特征反映了TLP基因在进化过程中的分化功能和特征,Or5与其他Ors最不同。此外,我们识别了TLP基因启动子中的25个顺式元件,并探索了TLP的多个新调控途径,包括光和激素反应。生物信息学和转录组学分析暗示了应激诱导的TLP基因的表达和可能的功能冗余。我们检测了6个OsTLP基因在水稻种子萌发后1~6天的表达水平,这些天变化最明显的是OsTLP10和OsTLP12。
UNASSIGNED:组合酵母双杂交系统和下拉测定,我们认为Or1的TLP基因在不同物种的种子萌发过程中可能具有相似的功能。总的来说,多物种TLP基因家族的综合分析结果为TLP基因家族的进化和功能提供了有价值的信息,为TLP基因的进一步应用和研究提供了依据。
UNASSIGNED: The highly conserved tubby-like proteins (TLPs) play key roles in animal neuronal development and plant growth. The abiotic stress tolerance function of TLPs has been widely explored in plants, however, little is known about comparative studies of TLPs within crops.
UNASSIGNED: Bioinformatic identification, phylogenetic analysis, Cis-element analysis, expression analysis, Cis-element analysis, expression analysis and so on were explored to analysis the
TLP gene family of multiple crops.
UNASSIGNED: In this study, a comprehensive analysis of
TLP genes were carried out in seven crops to explore whether similar function of TLPs in rice could be achieved in other crops. We identified 20, 9, 14, 11, 12, 35, 14 and 13 TLP genes in Glycine max, Hordeum vulgare, Sorghum bicolor, Arabidopsis thaliana, Oryza sativa Japonica, Triticum aestivum, Setaria italic and Zea mays, respectively. All of them were divided into two groups and ten orthogroups (Ors) based on amino acids. A majority of
TLP genes had two domains, tubby-like domain and F-box domain, while members of Or5 only had tubby-like domain. In addition, Or5 had more exons and shorter DNA sequences, showing that characteristics of different Ors reflected the differentiated function and feature of
TLP genes in evolutionary process, and Or5 was the most different from the other Ors. Besides, we recognized 25 cis-elements in the promoter of TLP genes and explored multiple new regulation pathway of TLPs including light and hormone response. The bioinformatic and transcriptomic analysis implied the stresses induced expression and possible functional redundancy of TLP genes. We detected the expression level of 6 OsTLP genes at 1 to 6 days after seed germination in rice, and the most obvious changes in these days were appeared in OsTLP10 and OsTLP12.
UNASSIGNED: Combined yeast two-hybrid system and pull down assay, we suggested that the TLP genes of Or1 may have similar function during seed germination in different species. In general, the results of comprehensive analysis of TLP gene family in multiple species provide valuable evolutionary and functional information of TLP gene family which are useful for further application and study of
TLP genes.