TGA转录因子(TFs)在拟南芥中表现出基本抗性,但对西红柿的病原体攻击的敏感性;然而,它们在大豆(Glycinemax)和大豆花叶病毒(SMV)中的作用尚不清楚。在这项研究中,从SMV超易感大豆NN1138-2中分离出27个TGA基因,命名为GmTGA1〜GmTGA27,它们分为七个系统发育组。GmTGAs的表达谱显示高表达基因主要集中在Ⅰ组,II,和VII在非诱导条件下,在27个GmTGA中,19对SMV诱导有反应。有趣的是,在进一步的瞬时N.benthamiana-SMV病理系统测定中,所有过表达的19个GmTGA均不促进接种叶片中的SMV感染,但除了一个没有功能外,它们表现出基础抗性。在18个功能中,GmTGA8和GmTGA19,具有相似的基序分布,核定位序列和相互作用蛋白,显示出对SMV感染的快速反应,并且在抑制SMV增殖方面比其他表现更好。这一发现表明,即使来自高易感来源,GmTGATF也可能支持对SMV的基础抗性。基因(GmTGA8、GmTGA19等。)具有对SMV的基本抗性,以及它们未来提高大豆对SMV抗性的潜力,有待探索。
TGA transcription factors (TFs) exhibit basal resistance in Arabidopsis, but susceptibility to a pathogen attack in tomatoes; however, their roles in soybean (Glycine max) to Soybean mosaic virus (SMV) are unknown. In this study, 27 TGA genes were isolated from a SMV hyper-susceptible soybean NN1138-2, designated GmTGA1~GmTGA27, which were clustered into seven phylogenetic groups. The expression profiles of GmTGAs showed that the highly expressed genes were mainly in Groups I, II, and VII under non-induction conditions, while out of the 27 GmTGAs, 19 responded to SMV-induction. Interestingly, in further transient N. benthamiana-SMV pathosystem assay, all the 19 GmTGAs overexpressed did not promote SMV infection in inoculated leaves, but they exhibited basal resistance except one without function. Among the 18 functional ones, GmTGA8 and GmTGA19, with similar motif distribution, nuclear localization sequence and interaction proteins, showed a rapid response to SMV infection and performed better than the others in inhibiting SMV multiplication. This finding suggested that GmTGA TFs may support basal resistance to SMV even from a hyper-susceptible source. What the mechanism of the genes (GmTGA8, GmTGA19, etc.) with basal resistance to SMV is and what their potential for the future improvement of resistance to SMV in soybeans is, are to be explored.