PDF(Pubmed)
Abstract：
TGA transcription factors belong to Group D of the bZIP transcription factors family and play vital roles in the stress response of plants. Brassica napus is an oil crop with rich economic value. However, a systematic analysis of TGA gene family members in B. napus has not yet been reported. In this study, we identified 39 full-length TGA genes in B. napus, renamed TGA1~TGA39. Thirty-nine BnTGA genes were distributed on 18 chromosomes, mainly located in the nucleus, and differences were observed in their 3D structures. Phylogenetic analysis showed that 39 BnTGA genes could be divided into five groups. The BnTGA genes in the same group had similar structure and motif compositions, and all the BnTGA genes had the same conserved bZIP and DOG1 domains. Phylogenetic and synteny analysis showed that the BnTGA genes had a close genetic relationship with the TGA genes of the Brassica juncea, and BnTGA11 and BnTGA29 may play an important role in evolution. In addition, qRT-PCR revealed that three genes (BnTGA14/17/23) showed significant changes in eight experimental materials after drought treatment. Meanwhile, it can be inferred from the results of drought treatment on different varieties of rapeseed that the stress tolerance of parental rapeseed can be transmitted to the offspring through hybridization. In short, these findings have promoted the understanding of the B. napus TGA gene family and will contribute to future research aimed at B. napus resistant breeding.
摘要：
TGA转录因子属于bZIP转录因子家族的D组，在植物的胁迫反应中起着至关重要的作用。甘蓝型油菜是一种具有丰富经济价值的油料作物。然而,对甘蓝型油菜TGA基因家族成员的系统分析尚未见报道。在这项研究中,我们在油菜中鉴定出39个全长TGA基因，更名为TGA1~TGA39。39个BnTGA基因分布在18条染色体上,主要位于细胞核,并在其3D结构中观察到差异。系统发育分析表明39个BnTGA基因可分为5组。同一组中的BnTGA基因具有相似的结构和基序组成，所有BnTGA基因都具有相同的保守bZIP和DOG1结构域。系统发育和同种学分析表明，BnTGA基因与芥菜属的TGA基因具有密切的遗传关系，BnTGA11和BnTGA29可能在进化中起重要作用。此外,qRT-PCR显示3个基因(BnTGA14/17/23)在干旱处理后的8个实验材料中表现出显著的变化。同时,从不同品种油菜的干旱处理结果可以推断,亲本油菜的抗逆性可以通过杂交传递给后代。总之,这些发现促进了对甘蓝型油菜TGA基因家族的理解，并将有助于未来针对甘蓝型油菜抗性育种的研究。
