最近,越来越多的报道描述了动物来源的病原体,这些病原体引起各种感染,并增加了它们向人类的传播。溶胆链球菌,牛链球菌/马链球菌复合体(SBSEC)的成员,是这些病原体之一,感染从哺乳动物到家禽的多种宿主,并且具有从致病性到食物发酵的广泛功能。由于胆溶菌引起包括菌血症在内的并发症,感染性心内膜炎,和人类的结肠直肠恶性肿瘤,重要的是调查它在各种宿主中的发生,包括鹅,以防止潜在的人畜共患传播。这项研究旨在调查临床健康和腹泻的鹅粪便中是否存在胆溶菌,密集和半密集地饲养,通过体外培养方法,通过PCR和基于序列的分子方法表征回收的分离株,并通过圆盘扩散和梯度测试方法确定其抗生素敏感性。为此,使用150个新鲜鹅粪便样品。对卵溶链球菌的培养阳性被确定为8%(12/150)。PCR分析鉴定出54.55%(n=6)的分离株为溶胆囊链球菌亚种。胆溶菌和45.45%(n=5)为胆溶菌亚种。巴氏杆菌.在16SrRNA序列和ERIC-PCR分析之后,卵黄S.亚种表现出相同的簇和带谱,可以很容易地彼此区分并进行克隆鉴定。氟苯尼考的敏感性高,青霉素,利福平,在分离物中检测到万古霉素,不管亚种的多样性。两个亚种对杆菌肽都表现出很高的抗性,克林霉素,多西环素,四环素,甲氧苄啶-磺胺甲恶唑,和红霉素和多种MDR谱,表明它们有可能成为超级细菌.来自Türkiye的第一份报告证明了鹅中gallolyticus亚种的出现。鉴于最近在蒂尔基耶的鹅产量和鹅肉消费量的增加,不应忽视鹅的发生,以防止人畜共患传播。
Recently, an increased number of reports have described pathogens of animal origin that cause a variety of infections and a rise in their transmission to humans. Streptococcus gallolyticus, a member of the Streptococcus bovis/Streptococcus equinus complex (SBSEC), is one of these pathogens and infects a wide range of hosts from mammals to poultry and has a broad functionality ranging from pathogenicity to food fermentation. As S. gallolyticus causes complications including bacteremia, infective endocarditis, and colorectal malignancy in humans, it is important to investigate its occurrence in various hosts, including geese, to prevent potential zoonotic transmissions. This study aimed to investigate the presence of S. gallolyticus in the droppings of clinically healthy and diarrheic geese, which were raised intensively and semi-intensively, by the in vitro culture method, characterize the isolates recovered by PCR and sequence-based molecular methods and determine their antibiotic susceptibility by the disk diffusion and gradient test methods. For this purpose, 150 samples of fresh goose droppings were used. Culture positivity for S. gallolyticus was determined as 8% (12/150). PCR analysis identified 54.55% (n = 6) of the isolates as S. gallolyticus subsp. gallolyticus and 45.45% (n = 5) as S. gallolyticus subsp. pasteurianus. Following the 16S rRNA sequence and ERIC-PCR analyses, S. gallolyticus subspecies exhibited identical cluster and band profiles that could be easily distinguished from each other and were clonally identified. High rates of susceptibility to florfenicol, penicillin, rifampicin, and vancomycin were detected among the isolates, regardless of the subspecies diversity. Both subspecies showed high levels of resistance to bacitracin, clindamycin, doxycycline, tetracycline, trimethoprim-sulfamethoxazole, and erythromycin and multiple MDR profiles, indicating their potential to become superbugs. This first report from Türkiye demonstrates the occurrence of the S. gallolyticus subspecies in geese. In view of the recent increase of geese production and the consumption of goose meat in Türkiye, the occurrence of S. gallolyticus in geese should not be ignored to prevent zoonotic transmission.