■这项研究的目的是确定丰富度,丰度,以及在ComarcaLagunera被蜱感染的流浪狗(犬类狼疮)中细菌的多样性,墨西哥北部,并确定其致病性和/或人畜共患潜力。
■收集12只狗的血样,提取了它们的脱氧核糖核酸。通过聚合酶链反应扩增了16S核糖体核糖酸基因的V3-V4区域。下一代测序(NGS)在MiSeqIllumina平台上进行,并使用对微生物生态学的定量见解对数据进行分析。
■操作分类单位导致23个门,54班,89个订单,189个家庭,586属,和620种细菌;其中,确定了64种具有致病性或人畜共患潜力的物种和/或细菌属,其中一些已在文献中报道为与公共卫生相关(吞噬细胞无性体,布鲁氏菌属。,梭菌属。,棒状杆菌,镰刀菌属。,Dietziaspp.,犬埃里希亚,坏死梭杆菌,Leptotrichiaspp.,分枝杆菌。,Paracocusspp.,和吉拉迪玫瑰单胞菌)。
■这项研究提供了有关墨西哥北部被壁虱寄生的流浪狗血液中壁虱传播疾病以及其他潜在的人畜共患疾病的患病率的相关信息。新的分子生物学和大规模NGS技术可能在研究和记录人类附近动物的细菌谱中发挥重要作用。
UNASSIGNED: The objectives of this study were to determine the richness, abundance, and diversity of bacteria in stray dogs (Canis lupus familiaris) infested by ticks in Comarca Lagunera, northern Mexico, and to establish their pathogenic and or/zoonotic potential.
UNASSIGNED: Blood samples from 12 dogs were collected, and their deoxyribonucleic acid was extracted. The V3-V4 region of the 16S ribosomal ribunocleic acid gene was amplified by polymerase chain reaction. Next-generation sequencing (NGS) was performed on a MiSeq Illumina platform, and the data were analyzed using quantitative insights into microbial ecology.
UNASSIGNED: The operational taxonomic units resulted in 23 phyla, 54 classes, 89 orders, 189 families, 586 genera, and 620 bacterial species; among them, 64 species and/or bacterial genera with pathogenic or zoonotic potential were identified, some of which have been reported in the literature as relevant to public health (Anaplasma phagocytophilum, Brucella spp., Clostridium spp., Corynebacterium affermentants, Cutibacterium spp., Dietzia spp., Ehrlichia canis, Fusobacterium necrophorum, Leptotrichia spp., Mycobacterium spp., Paracoccus spp., and Roseomonas gilardii).
UNASSIGNED: This research offers relevant information on the prevalence of tick-borne diseases as well as other potential zoonotic diseases in the blood of stray dogs parasitized by ticks in northern Mexico. New molecular biology and massive NGS techniques may play an important role in the study and documentation of bacterial profiles from animals in close proximity to humans.