The rapid proliferation of the halophilic pathogen Vibrio parahaemolyticus poses a severe health hazard to halobios and significantly impedes intensive mariculture. This study aimed to evaluate the potential application of gliding arc discharge plasma (GADP) to control the infection of Vibrio parahaemolyticus in mariculture. This study investigated the inactivation ability of GADP against Vibrio parahaemolyticus in artificial seawater (ASW), changes in the water quality of GADP-treated ASW, and possible inactivation mechanisms of GADP against Vibrio parahaemolyticus in ASW. The results indicate that GADP effectively inactivated Vibrio parahaemolyticus in ASW. As the volume of ASW increased, the time required for GADP sterilization also increased. However, the complete sterilization of 5000 mL of ASW containing Vibrio parahaemolyticus of approximately 1.0 × 104 CFU/mL was achieved within 20 min. Water quality tests of the GADP-treated ASW demonstrated that there were no significant changes in salinity or temperature when Vibrio parahaemolyticus (1.0 ×104 CFU/mL) was completely inactivated. In contrast to the acidification observed in plasma-activated water (PAW) in most studies, the pH of ASW did not decrease after treatment with GADP. The H2O2 concentration in the GADP-treated ASW decreased after post-treatment. The NO2-concentration in the GADP-treated ASW remained unchanged after post-treatment. Further analysis revealed that GADP induced oxidative stress in Vibrio parahaemolyticus, which increased cell membrane permeability and intracellular ROS levels of Vibrio parahaemolyticus. This study provides a viable solution for infection with the halophilic pathogen Vibrio parahaemolyticus and demonstrates the potential of GADP in mariculture.

Nanomaterials used as novel antimicrobial agents are developed to combat wide-spectrum bacteria as well as alleviate the problem of bacterial resistance to traditional antibiotics. Two-dimensional (2D) nanomaterials are regarded as promising and widely applicable wide-spectrum antibacterial nanoagents due to their outstanding characteristics, including large surface area, stable structure, good biocompatibility, and cheap raw materials, especially excellent photocatalytic or photothermal properties. The advantages of less resistance, rapid sterilization, local action mode, and non-invasive make 2D nanomaterials with photoinduced property stand out in novel sterilization agents. Here, we systematically summarize various kinds of 2D nanomaterials that were used in photoinduced antibacterial field and introduce the synthesis methods and physicochemical properties related to antibacterial capability. Besides, the sterilization mechanisms of different 2D nanomaterials are also introduced in detail including a direct temperature generation, ROS production or ROS-independent oxidative stress, or various synergistic antibacterial modes. Lastly, we have also discussed the current problems of 2D nanomaterials as light-inducing antibacterial agents and their future application prospects in the fields of biomedicine, sewage treatment, food, and other application scenarios. We envision that this review will assist investigators with quick and in-depth understanding of the latest progress in 2D nanomaterials for photoinduced sterilization, inspiring the development of multiply strategies to design and synthesize novel 2D materials with strong bactericidal capability and high biological safety, and expand 2D materials with photoinduced sterilization to more application scenarios.