Abstract：
The rapid proliferation of the halophilic pathogen Vibrio parahaemolyticus poses a severe health hazard to halobios and significantly impedes intensive mariculture. This study aimed to evaluate the potential application of gliding arc discharge plasma (GADP) to control the infection of Vibrio parahaemolyticus in mariculture. This study investigated the inactivation ability of GADP against Vibrio parahaemolyticus in artificial seawater (ASW), changes in the water quality of GADP-treated ASW, and possible inactivation mechanisms of GADP against Vibrio parahaemolyticus in ASW. The results indicate that GADP effectively inactivated Vibrio parahaemolyticus in ASW. As the volume of ASW increased, the time required for GADP sterilization also increased. However, the complete sterilization of 5000 mL of ASW containing Vibrio parahaemolyticus of approximately 1.0 × 104 CFU/mL was achieved within 20 min. Water quality tests of the GADP-treated ASW demonstrated that there were no significant changes in salinity or temperature when Vibrio parahaemolyticus (1.0 ×104 CFU/mL) was completely inactivated. In contrast to the acidification observed in plasma-activated water (PAW) in most studies, the pH of ASW did not decrease after treatment with GADP. The H2O2 concentration in the GADP-treated ASW decreased after post-treatment. The NO2-concentration in the GADP-treated ASW remained unchanged after post-treatment. Further analysis revealed that GADP induced oxidative stress in Vibrio parahaemolyticus, which increased cell membrane permeability and intracellular ROS levels of Vibrio parahaemolyticus. This study provides a viable solution for infection with the halophilic pathogen Vibrio parahaemolyticus and demonstrates the potential of GADP in mariculture.
摘要：
嗜盐病原体副溶血性弧菌的快速增殖对鱼类构成严重的健康危害，并严重阻碍了集约化的海水养殖。本研究旨在评估滑动电弧放电等离子体（GADP）在控制海水养殖中副溶血性弧菌感染中的潜在应用。这项研究调查了GADP对人工海水（ASW）中副溶血性弧菌的灭活能力，经GADP处理的ASW的水质变化，以及GADP对ASW中副溶血性弧菌的可能灭活机制。结果表明，GADP可有效灭活ASW中的副溶血性弧菌。随着ASW体积的增加，GADP灭菌所需的时间也增加了。然而,在20分钟内完成了含有约1.0×104CFU/mL副溶血性弧菌的5000mLASW的完全灭菌。经GADP处理的ASW的水质测试表明，当副溶血性弧菌（1.0×104CFU/mL）完全灭活时，盐度或温度没有显着变化。与大多数研究中在血浆活化水（PAW）中观察到的酸化相反，用GADP处理后，ASW的pH值没有降低。后处理后，GADP处理的ASW中的H2O2浓度降低。后处理后，GADP处理的ASW中的NO2浓度保持不变。进一步分析显示，GADP诱导副溶血性弧菌的氧化应激，增加副溶血弧菌的细胞膜通透性和细胞内ROS水平。这项研究为嗜盐病原体副溶血性弧菌的感染提供了可行的解决方案，并证明了GADP在海水养殖中的潜力。
