PEG包覆的铁氧体纳米粒子Co0.2Mn0.6Zn0.2Fe2O4(X1),Co0.4Mn0.4Zn0.2Fe2O4(X2),通过共沉淀法合成了Co0.6Mn0.2Zn0.2Fe2O4(X3)。用XRD对纳米粒子进行了表征,拉曼,VSM,XPS,和TEM。使用交变磁场(AMF)测定PEG包被的纳米颗粒的磁性热疗效率(MH)。在水介质中,2mg/mL的X2纳米颗粒显示出最高的饱和磁化强度和比吸收率(SAR)值为245.2W/g。基于这些属性,进一步评估X2纳米颗粒在495.25kHz频率和350G强度的AMF下对HCT116细胞的抗增殖活性,使用MTT,菌落形成,伤口愈合试验,和流式细胞术分析,以确定细胞活力,克隆性质,细胞迁移能力,以及在HCT116细胞中AMF处理后HCT116细胞的细胞死亡,分别。我们观察到细胞活力的显着抑制(未经处理的对照与2%50%为AMF),集落形成能力(未经处理的对照与530个细胞/集落用于AMF的220个细胞/菌落),消除细胞迁移(未经治疗的对照与100%伤口闭合用于AMF的5%伤口闭合),和诱导凋亡介导的细胞死亡(未经处理的对照与7.5%相对于AMF处理后未处理的对照细胞,AMF为24.7%)的HCT116细胞。总的来说,这些结果表明,与未处理的细胞相比,用AMF处理的PEG涂层(CoMnZn-Fe2O4)混合铁氧体纳米颗粒对HCT116细胞具有明显的抗增殖作用,表明Co0.4Mn0.4Zn0.2Fe2O4纳米颗粒靶向结直肠癌细胞的有希望的抗增殖潜力。此外,这些结果提供了有吸引力的证据,证明使用MH的铁氧体基纳米粒子可以作为潜在的抗癌药物,在未来针对结直肠癌和其他癌症的研究中,需要在临床前模型中进一步评估.
The PEG-coated ferrite nanoparticles Co0.2Mn0.6Zn0.2Fe2O4 (X1), Co0.4Mn0.4Zn0.2Fe2O4 (X2), and Co0.6Mn0.2Zn0.2Fe2O4 (X3) were synthesized by the coprecipitation method. The nanoparticles were characterized by XRD, Raman, VSM, XPS, and TEM. The magnetic hyperthermia efficiency (MH) was determined for PEG-coated nanoparticles using an alternating magnetic field (AMF). X2 nanoparticles displayed the highest saturation magnetization and specific absorption rate (SAR) value of 245.2 W/g for 2 mg/mL in a water medium. Based on these properties, X2 nanoparticles were further evaluated for antiproliferative activity against HCT116 cells at an AMF of 495.25 kHz frequency and 350 G strength, using MTT, colony formation, wound healing assays, and flow cytometry analysis for determining the cell viability, clonogenic property, cell migration ability, and cell death of HCT116 cells upon AMF treatment in HCT116 cells, respectively. We observed a significant inhibition of cell viability (2% for untreated control vs. 50% for AMF), colony-forming ability (530 cells/colony for untreated control vs. 220 cells/colony for AMF), abrogation of cell migration (100% wound closure for untreated control vs. 5% wound closure for AMF), and induction of apoptosis-mediated cell death (7.5% for untreated control vs. 24.7% for AMF) of HCT116 cells with respect to untreated control cells after AMF treatment. Collectively, these results demonstrated that the PEG-coated (CoMnZn-Fe2O4) mixed ferrite nanoparticles upon treatment with AMF induced a significant antiproliferative effect on HCT116 cells compared with the untreated cells, indicating the promising antiproliferative potential of the Co0.4Mn0.4Zn0.2Fe2O4 nanoparticles for targeting colorectal cancer cells. Additionally, these results provide appealing evidence that ferrite-based nanoparticles using MH could act as potential anticancer agents and need further evaluation in preclinical models in future studies against colorectal and other cancers.