■本研究旨在通过Nrf2,NOX4,超氧化物歧化酶,甲基苯丙胺戒断后大鼠肺部的丙二醛基因。
■将40只雄性Wistar大鼠随机分为5组(每组8只),接受甲基苯丙胺管理(六周,5mg/kgip,每天一次),然后是21天的停药期。以每天25g/kg的剂量向大鼠补充NBS超级食物持续六周。训练方案是每天30分钟的连续训练(跑步机跑步),一周五天,六个星期。该方案从最初5分钟的3米/分钟的步速升级,在接下来的5分钟内达到5米/分钟,会议剩余时间以8米/分钟的速度结束,全部以0°倾斜。进行单因素方差分析以分析Nrf2,NOX4,MDA,和大鼠肺组织中的SOD。
■结果表明,在接受持续训练和NBS超级食物补充的实验组中,与对照组相比,Nrf2基因的表达显着升高(P<0.05),而NOX4,MDA,与对照组相比,SOD基因的表达显着下降(P<0.05)。
■一般来说,运动干预和NBS超级食物补充剂,甲基苯丙胺戒断后单独或联合使用时,能增强肺部氧化应激状态。
UNASSIGNED: This study aimed to investigate the effects of six weeks of continuous training and Nutrition Bio-shield (NBS) Superfood Supplementation on the state of oxidative stress by the expression of Nrf2, NOX4, superoxide dismutase, and malondialdehyde genes in the lungs of rats after methamphetamine withdrawal.
UNASSIGNED: Forty male Wistar rats were randomly divided into five groups (n = 8, per group), undergoing methamphetamine administration (six weeks, 5 mg/kg ip, and once per day) followed by a 21-day withdrawal period. The rats were supplemented NBS superfood at a dosage of 25 g/kg per day for six weeks. The training protocol was 30 minutes of daily continuous training (treadmill running), five days a week for six weeks. The regimen escalated from a pace of 3 m/min for the initial 5 minutes, to 5 m/min for the following 5 minutes, culminating at 8 m/min for the remainder of the session, all at a 0° incline. A one-way analysis of variance was performed to analyze the gene expression of Nrf2, NOX4, MDA, and
SOD in the lungs tissue of rats.
UNASSIGNED: The results indicated that, in the experimental groups which underwent continuous training and NBS Superfood supplementation, the expression of the Nrf2 gene exhibited a significant elevation compared to the control group (P < 0.05), while the NOX4, MDA, and
SOD genes expression exhibited a significant decline in comparison to the control group (P < 0.05).
UNASSIGNED: In general, both exercise interventions and NBS superfood supplementation, when employed separately or in combination after methamphetamine withdrawal, can enhance the state of oxidative stress in the lung.