PDF(Pubmed)
Abstract：
The faba bean, a significant cool-season edible legume crop, is susceptible to drought during the germination stage. Research regarding the genetic regulation of drought tolerance throughout this stage in the faba bean is limited. The differentially expressed proteins (DEPs) in faba beans between the drought-tolerant variety C105 and the drought-sensitive variant E1 during seed germination were identified in this work, accomplished through isobaric tags for relative and absolute quantitation (iTRAQ) analysis. A total of 3827 proteins were identified in the two varieties of germinating seeds. Compared to those of variety E1, an increase in 108 DEPs and a decrease in 61 DEPs were observed in variety C105 under drought. Conversely, in the control group, variety C105 showed 108 significantly upregulated DEPs and 55 significantly downregulated DEPs. GO and KEGG analyses showed that the DEPs associated with glutathione metabolism and protein processing demonstrated significant increases in response to drought stress. Protein-protein interaction (PPI) analysis unveiled three closely connected functional modules of protein translation, DNA replication, and post-translational modification, originating from 22 DEPs derived from the germination period of two varieties under drought stress. To verify the proteomic function, we selected three differentially expressed protein coding genes, which were overexpressed or silenced in tobacco, thereby enhancing the drought resistance of tobacco. This was accompanied via altered levels of superoxide dismutase or peroxidase in transgenic plants under drought stress. The possible mechanism for drought tolerance in germinating seeds of faba bean involves increasing protein translation, decreasing DNA replication, and modifying chromatin. These findings offer invaluable insights into the reaction mechanism in response to drought stress in faba beans. The identified DEPs could be utilized in faba bean breeding initiatives to manage drought.
摘要：
蚕豆，一种重要的冷季食用豆类作物，在发芽阶段易受干旱影响。关于蚕豆在整个阶段耐旱性的遗传调控的研究有限。在这项工作中，鉴定了耐旱品种C105和干旱敏感变体E1在种子萌发过程中在蚕豆中的差异表达蛋白（DEP），通过等量异位标签进行相对和绝对定量(iTRAQ)分析。在两种发芽种子中总共鉴定出3827种蛋白质。与E1品种相比，干旱下C105品种增加了108个DEP，减少了61个DEP。相反，在对照组中，品种C105显示108显着上调DEP和55显着下调DEP。GO和KEGG分析表明，与谷胱甘肽代谢和蛋白质加工相关的DEP表现出对干旱胁迫的响应显着增加。蛋白质-蛋白质相互作用（PPI）分析揭示了蛋白质翻译的三个紧密相连的功能模块，DNA复制,和翻译后修饰，起源于22个DEP，来自两个品种在干旱胁迫下的发芽期。为了验证蛋白质组功能，我们选择了三个差异表达的蛋白质编码基因，在烟草中过度表达或沉默，从而增强烟草的抗旱性。这伴随着干旱胁迫下转基因植物中超氧化物歧化酶或过氧化物酶水平的改变。蚕豆发芽种子耐旱性的可能机制涉及增加蛋白质翻译，减少DNA复制，和修饰染色质。这些发现为蚕豆应对干旱胁迫的反应机制提供了宝贵的见解。确定的DEP可用于蚕豆育种计划以管理干旱。
