未经证实:肌肉减少症是一种与年龄相关的骨骼肌功能障碍综合征,缺乏有效的治疗方法。在年轻时最大限度地提高肌肉力量可能是预防老年人肌肉减少症的一种有希望的方法。植物分子葛根素已广泛用于临床实践,并有报道通过直接靶向骨骼肌纤维来增加骨骼肌的能量代谢。然而,葛根素的生物利用度很差,近93%的葛根素会留在肠道中直到排泄。因此,我们假设葛根素可能调节肠道菌群,从而改善成人骨骼肌的力量和/或质量.
UNASSIGNED:将23个月大的雄性SpragueDawley大鼠按平均体重分为两组,葛根素组(葛根素溶于0.5%CMC-Na,150毫克/千克/天,N=10),和对照组(等体积0.5%CMC-Na,N=10)。治疗持续8周。肌肉重量,肌纤维类型和横截面积(CSA),测量离体肌肉收缩测试和握力。采用16SrDNA测序来评估盲肠内容物样品中的肠道微生物群组成。采用气相色谱-质谱法分析盲肠和血清中的短链脂肪酸(SCFAs)。还检测了骨骼肌中的三磷酸腺苷(ATP)浓度。采用皮尔逊相关性分析SCFA之间的关系,ATP浓度和肌肉功能。
未经批准:葛根素治疗后,握力,特定的抽搐力,比目鱼肌(SOL)和趾长伸肌(EDL)的强直力明显高于对照组。葛根素组EDL中II型肌纤维的百分比和CSA高于对照组。葛根素处置明显转变了肠道微生物构成。两种SCFA生产微生物群,Peptococaceae和Closteridiales家族,葛根素组明显高于对照组,而Prevotellaceae/拟杆菌科的比率(P/B),肌肉萎缩指标,葛根素组较低。不出所料,SCFA的浓度之间存在显著的线性相关性,包括盲肠总SCFA,血清正丁酸和总SCFA,和骨骼肌的力量和功能,包括SOL和EDL的抽搐力和强直力,以及前肢的握力。
未经批准:总而言之,葛根素改善了幼年大鼠前肢握力和肌肉收缩功能。潜在的机制可能包括葛根素通过调节肠道微生物群增加SCFAs的产生,增强ATP合成和骨骼肌力量。本文的翻译潜力:我们的研究发现,临床使用的植物分子葛根素具有改善年轻成年大鼠骨骼肌力量的潜力。由于葛根素具有长期的临床经验和良好的安全性,它可能是开发肌肉强化剂的潜在候选者。
UNASSIGNED: Sarcopenia is an age-related skeletal muscle dysfunction syndrome that is lacking validated treatments. Maximizing muscle strength in young adulthood may be a promising way to prevent sarcopenia in the elderly. The phytomolecule puerarin has been extensively used in clinical practice and reported to increase energy metabolism in skeletal muscle by directly targeting the skeletal muscle fiber. However, the bioavailability of puerarin is very poor, and almost 93% of puerarin stays in the intestine until excretion. Therefore, we hypothesize that puerarin may regulate gut microbiota to improve skeletal muscle strength and/or mass in adults.
UNASSIGNED: Twenty three-month old male Sprague Dawley rats were divided into two groups according to average weights, puerarin group (puerarin dissolved in 0.5% CMC-Na, 150 mg/kg/day, N = 10), and control group (equal volume 0.5% CMC-Na, N = 10). The treatment lasted for 8 weeks. Muscle weight, muscle fiber types and cross-sectional area (CSA), ex vivo muscle contraction test and grip strength were measured. 16S rDNA sequencing was employed to evaluate the gut microbiota composition in the sample of cecal content. Short-chain fatty acids (SCFAs) in cecal and serum were analyzed by gas chromatography-mass spectrometry. Adenosine triphosphate (ATP) concentration in skeletal muscle was also detected. Pearson\'s correlation was used to analyze the relations between SCFAs, ATP concentration and muscle function.
UNASSIGNED: After puerarin treatment, grip strength, the specific twitch force, and the tetanic forces in the soleus (SOL) and extensor digitorum longus (EDL) muscle were significantly higher than those of the control group. The percentage and CSA of type II muscle fiber in EDL was higher in the puerarin group than those in the control group. Puerarin treatment significantly changed the gut microbial constitutes. Two SCFAs-productive microbiota, the families Peptococcaceae and Closteridiales, were significantly higher in the puerarin group than those in the control group, while the ratio of Prevotellaceae/Bacteroidaceae (P/B), a muscle atrophy indicator, was lower in the puerarin group. As expected, there were significant linear correlations between the concentrations of SCFAs, including cecal total SCFAs, serum n-butyric acid and total SCFAs, and skeletal muscle strength and function, including the twitch force and tetanic force of SOL and EDL, as well as the forelimb grip strength.
UNASSIGNED: In conclusion, puerarin improved the forelimb grip strength and muscle contraction function in young adult rats. The underlying mechanism may include that puerarin increased SCFAs production by regulating gut microbiota, augmented ATP synthesis and skeletal muscle strength. The translational potential of this article : Our study finds that a clinical used phytomolecule puerarin has the potential of improving skeletal muscle strength in young adult rats. As puerarin has long-term clinical experience and shows good safety, it might be a potential candidate for developing muscle strengthening agents.