背景:非甾体抗炎药(NSAID)通过抑制环氧合酶(COX)酶而具有镇痛和抗炎特性。关于NSAIDs是否影响与肌肉适应和运动相关的信号,存在矛盾的证据,一些研究发现通过AKT-mTOR通路减少肌肉蛋白合成信号。卫星细胞信号的变化,减少肌肉蛋白质降解,和细胞增殖的减少。在这项研究中,我们确定了单一最大剂量的氟比洛芬(FLU),塞来昔布(CEL),布洛芬(IBU),或安慰剂(PLA)会影响短期肌肉信号对补强运动的反应。
方法:这是一个随机分组,双面蒙面,交叉设计,其中12名参与者进行了四次屈光度运动,包括10组10次屈光度跳跃,1RM为40%。锻炼前两小时,参与者服用单剂量的塞来昔布(CEL200mg),IBU(800毫克),FLU(100毫克)或PLA与食物。运动前和运动后3小时从股外侧肌收集肌肉活检样本。使用重复测量(RM)方差分析分析数据,方差分析,或者弗里德曼测试.在p<0.05时考虑显著性。
结果:我们发现对PTSG1,PTSG2,MYC,TBP,RPLOP,MYOD1,Pax7,MYOG,Atrogin-1或MURF1(全部,p>0.05)。我们还发现,通过mTORS2441,mTORS2448,RPS6235/236,RPS240/244,4EBP1,ERK1/2,p38T180/182的磷酸化状态测量,对AKT-mTOR信号或MAPK信号没有治疗作用。p>0.05)。然而,我们确实发现与FLU相比,PLA中MNK1T197/202之间存在显着差异(p<0.05)。
结论:单个,最大剂量的IBU,CEL,或者运动前服用的FLU不会影响肌肉蛋白合成的信号,蛋白质降解,或核糖体生物发生在一次定量训练后三小时。
BACKGROUND: Non-steroidal anti-inflammatory drugs (NSAIDs) possess analgesic and anti-inflammatory properties by inhibiting cyclooxygenase (COX) enzymes. Conflicting evidence exists on whether NSAIDs influence signaling related to muscle adaptations and exercise with some research finding a reduction in muscle protein synthesis signaling via the AKT-mTOR pathway, changes in satellite cell signaling, reductions in muscle protein degradation, and reductions in cell proliferation. In this study, we determined if a single maximal dose of flurbiprofen (FLU), celecoxib (CEL), ibuprofen (IBU), or a placebo (PLA) affects the short-term muscle signaling responses to plyometric exercise.
METHODS: This was a block randomized, double-masked, crossover design, where 12 participants performed four plyometric exercise bouts consisting of 10 sets of 10 plyometric jumps at 40% 1RM. Two hours before exercise, participants consumed a single dose of celecoxib (CEL 200 mg), IBU (800 mg), FLU (100 mg) or PLA with food. Muscle biopsy samples were collected before and 3-h after exercise from the vastus lateralis. Data were analyzed using a repeated measures (RM) ANOVA, ANOVA, or a Friedman test. Significance was considered at p < 0.05.
RESULTS: We found no treatment effects on the mRNA expression of PTSG1, PTSG2, MYC, TBP, RPLOP, MYOD1, Pax7, MYOG, Atrogin-1, or MURF1 (all, p > 0.05). We also found no treatment effects on AKT-mTOR signaling or MAPK signaling measured through the phosphorylation status of mTORS2441, mTORS2448, RPS6 235/236, RPS 240/244, 4EBP1, ERK1/2, p38 T180/182 normalized to their respective total abundance (all, p > 0.05). However, we did find a significant difference between MNK1 T197/202 in PLA compared to FLU (p < .05).
CONCLUSIONS: A single, maximal dose of IBU, CEL, or FLU taken prior to exercise did not affect the signaling of muscle protein synthesis, protein degradation, or ribosome biogenesis three hours after a plyometric training bout.