这项工作旨在通过使用响应面方法(RSM)和Box-Benken模型的优化过程,评估作为AgNPs绿色合成还原剂对HT的影响。在HT上合成AgNPs的最佳条件包括6.15小时的反应时间。50℃的反应温度和稀释的刺梨叶提取物还原AgNO3的比例为50.37mL/mg。在最优条件下,还原反应收率达到77.54%,接近理论值的76.97%。此外,形态学,密度,并利用紫外可见光谱法测定了HT层表面AgNPs的特征,场发射扫描电子显微镜,高分辨率透射电子显微镜,选定区域衍射,X射线衍射,动态光散射,红外光谱,荧光发射光谱,Brunauer-Emmett-Teller方法。在HT表面成功合成了平均粒径为13.0±1.1nm的球形AgNPs。有趣的是,HT-Ag杂化材料能强烈抑制大肠杆菌的生长,金黄色葡萄球菌以及两种抗生素抗性细菌菌株,StutzeriB27和抗生素抗性大肠杆菌。总的来说,HT-Ag杂化材料具有很好的应用前景。
Hydrotalcite-silver (HT-Ag) nanoparticles have been involved in various daily crucial applications, such as antibacterial, photocatalytic, adsorption, etc. There are many approaches to synthesizing silver nanoparticles (AgNPs) decorated on hydrotalcite (HT) surface and the most used approach is using a strong reducing agent. Thus, affordable but effective \"green\" reducing agents - Syzygium nervosum leaf extract, are taken into account in this work to solve several issues related to chemical reducing agents. This work aimed to assess the effect of Syzygium nervosum leaf extract as a reducing agent for green synthesis of AgNPs on HT through an optimizing process using response surface methodology (RSM) and the Box-Benken model. The optimal conditions for the synthesis of AgNPs on HT include a reaction time of 6.15 hours, a reaction temperature of 50 °C, and the ratio of diluted Syzygium nervosum leaf extract to reduce AgNO3 of 50.37 mL/mg. Under the optimal conditions, the yield of the reduction reaction reached 77.54 %, close to the theoretical value of 76.97 %. The optimization model was suitable for the experiment data. Besides, the morphology, density, and characteristics of AgNPs on the surface of HT layers have been determined by using Ultraviolet-visible spectroscopy, Field emission scanning electron microscopy (FESEM), High-resolution transmission electron microscopy (HR-TEM), selected area diffraction, X-ray diffraction, Dynamic light scattering (DLS), Infrared (IR) spectroscopy, Fluorescence emission spectroscopy (FE), Brunauer-Emmett-Teller (BET) methods. The spherical AgNPs were synthesized successfully on the surface of HT with the average particle size of 13.0±1.1 nm. Interestingly, HT-Ag hybrid materials can inhibit strongly the growth of E. coli, S. aureus as well as two antibiotic resistance bacterial strains, P. stutzeri B27, and antibiotic resistance E. coli. Especially, the antibacterial activity quantification and durability of the HT-Ag hybrid materials were also tested. Overall, the HT-Ag hybrid materials are very promising for application in material science and biomedicine fields.